Dataset Information

Salmon fast growing muscle LC-MSMS

ABSTRACT: Fast growth is vital to the commercial production of fish and has been achieved gradually, through generations of selective breeding, and instantaneously, through growth hormone (GH) transgenesis (GHT). The proteomic basis for these differing routes towards a largely common higher phenotype is poorly characterized, as are associated implications for fish health parameters. We addressed this knowledge gap in coho salmon (Oncorhynchus kisutch) testing two hypotheses: i) that selective breeding and GHT are underpinned by both parallel and non-parallel changes in growth pathways compared to wild-type, and ii) that rapidly growing fish have less scope to allocate energetic resources into immune function. Skeletal muscle was studied, owing to its central role in growth and energetic storage. We compared the proteomes of GHT and growth-selected domesticated strains with wild-type animals following injections with PBS (control) and Poly I:C (to mimic viral infection). Hybrid quadrupole-Orbitrap mass-spectrometry using a label-free analysis revealed large changes in the proteome of GHT and growth-selected strains that were strikingly non-overlapping, with surprisingly few parallel changes. GHT was characterized by a focal upregulation of translation systems, while growth-selected fish presented a larger and more diverse set of changes, consistent with genetic alterations influencing an array of metabolic and cellular pathways. Contrasting past studies examining mRNA levels, many proteomic changes distinguished the growth-selected strain from both GHT and wild fish. Contrasting our second hypothesis, Poly I:C had little detectable effect on the muscle proteome. This investigation reveals that highly distinct proteome profiles can explain outwardly similar growth phenotypes, improving our understanding of how fast growth is achieved in animal strains created by humans.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Oncorhynchus Kisutch

TISSUE(S): Skeletal Muscle Fiber

SUBMITTER: Dwight Causey

LAB HEAD: Daniel J. Macqueen

PROVIDER: PXD009537 | Pride | 2018-09-24

REPOSITORIES: Pride

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Publications

Proteomic comparison of selective breeding and growth hormone transgenesis in fish: Unique pathways to enhanced growth.

Causey Dwight R DR Kim Jin-Hyoung JH Stead David A DA Martin Samuel A M SAM Devlin Robert H RH Macqueen Daniel J DJ

Journal of proteomics 20180825

In fish used for food production and scientific research, fast growth can be achieved via selective breeding or induced instantaneously via growth hormone (GH) transgenesis (GHT). The proteomic basis for these distinct routes towards a similar higher phenotype remains uncharacterized, as are associated implications for health parameters. We addressed this knowledge gap using skeletal muscle proteomics in coho salmon (Oncorhynchus kisutch), hypothesising that i) selective breeding and GHT are und ...[more]

PMID: 30153513

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