Proteomics

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Synechocystis sp. proteome on differnt light conditions


ABSTRACT: Cyanobacteria are an integral part of the Earth’s biogeochemical cycles and a promising resource for the synthesis of renewable bioproducts from atmospheric CO2. Sustainable industrial applications, however, are still scarce and the true limits of phototrophic production remain unknown. One of the limitations of further progress is our insufficient understanding of the quantitative changes in photoautotrophic metabolism that occur during growth in dynamic environments. Uncovering principles of allocation of the key metabolites or proteins as a response to changing environment can provide significant advances in our understanding of phototrophic production limits. However, resolving such complex question requires multidisciplinary approach combining highly-controlled cyanobacteria cultivation coupled to quantitative analyses of specific cellular components. The following datase represents proteomic analysis of the model cyanobacterium Synechocystis sp. PCC 6803 that was cultivated in the well controlled environment of laboratory-scale photobioreactors in the quasi-continuous regime that allowed for maintaining the cells in the exponential growth phase. The sampels were taken under light-limited (25 - 220 µmol(photons) m-2 s-1), optimal (440 µmol(photons) m-2 s-1) and photoinhibited growth (1100 µmol(photons) m-2 s-1). Each culture was sampled in five biological replicates.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Synechocystis Sp.

TISSUE(S): Cell Culture

SUBMITTER: Gereon Poschmann  

LAB HEAD: Gereon Poschmann

PROVIDER: PXD009626 | Pride | 2019-01-07

REPOSITORIES: Pride

Dataset's files

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QX06504.raw Raw
QX06505.raw Raw
QX06506.raw Raw
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Publications


Phototrophic microorganisms are promising resources for green biotechnology. Compared to heterotrophic microorganisms, however, the cellular economy of phototrophic growth is still insufficiently understood. We provide a quantitative analysis of light-limited, light-saturated, and light-inhibited growth of the cyanobacterium <i>Synechocystis</i> sp. PCC 6803 using a reproducible cultivation setup. We report key physiological parameters, including growth rate, cell size, and photosynthetic activi  ...[more]

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