Proteomics

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Improving silkworm genome annotation using a proteogenomics approach

ABSTRACT: We collected a total of 9.8 million mass spectra generated in the laboratory from the proteomics analyses of different silkworm tissues, including the posterior silk gland (PSG) 30, middle silk gland 31, ovary and testis 32, head 33, brain, prothoracic glands, subesophageal ganglion 34, hemolymph 35, fat body 36 and embryo 37,38 of domestic silkworm, and the posterior silk gland of wild silkworm 39.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Bombyx Mori (silk Moth)

TISSUE(S): Blood

SUBMITTER: Boxiong Zhong  

LAB HEAD: Boxiong Zhong

PROVIDER: PXD009697 | Pride | 2019-07-01

REPOSITORIES: Pride

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TheproteinsidentifiedintheproteomicsanalysisProteinFDR1.rar Other
ZB9-Drosophila.dat Other
ZB9-bombyx.dat Other
ZB9.dat Other
blood.thefifthdayof5thinstarlarva.raw.rar Raw
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Publications

Improving Silkworm Genome Annotation Using a Proteogenomics Approach.

Ye Xiaogang X   Tang Xiaoli X   Wang Xiaoxiao X   Che Jiaqian J   Wu Meiyu M   Liang Jianshe J   Ye Lupeng L   Qian Qiujie Q   Li Jianying J   You Zhengying Z   Zhang Yuyu Y   Wang Shaohua S   Zhong Boxiong B  

Journal of proteome research 20190702 8

The silkworm genome has been deeply sequenced and assembled, but accurate genome annotation, which is important for modern biological research, remains far from complete. To improve silkworm genome annotation, we carried out a proteogenomics analysis using 9.8 million mass spectra collected from different tissues and developmental stages of the silkworm. The results confirmed the translational products of 4307 existing gene models and identified 1701 novel genome search-specific peptides (GSSPs)  ...[more]

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