Dataset Information

Proteomic analysis of Plasmodium falciparum oocyst sporozoites

ABSTRACT: Proteomic analysis of Plasmodium falciparum oocyst sporozoites

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Plasmodium Falciparum Nf54

DISEASE(S): Malaria

SUBMITTER: Kristian Swearingen

LAB HEAD: Robert L. Moritz

PROVIDER: PXD009728 | Pride | 2019-10-02

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	2016-10-14_KES_Pf_oo-spz_01_A.pep.xml	Pepxml
	2016-10-14_KES_Pf_oo-spz_01_A.raw	Raw
	2016-10-14_KES_Pf_oo-spz_01_B.pep.xml	Pepxml
	2016-10-14_KES_Pf_oo-spz_01_B.raw	Raw
	2016-10-14_KES_Pf_oo-spz_01_C.pep.xml	Pepxml

Items per page:

1 - 5 of 152

Publications

Transcriptomics and proteomics reveal two waves of translational repression during the maturation of malaria parasite sporozoites.

Lindner Scott E SE Swearingen Kristian E KE Shears Melanie J MJ Walker Michael P MP Vrana Erin N EN Hart Kevin J KJ Minns Allen M AM Sinnis Photini P Moritz Robert L RL Kappe Stefan H I SHI

Nature communications 20191031 1

Plasmodium sporozoites are transmitted from infected mosquitoes to mammals, and must navigate the host skin and vasculature to infect the liver. This journey requires distinct proteomes. Here, we report the dynamic transcriptomes and proteomes of both oocyst sporozoites and salivary gland sporozoites in both rodent-infectious Plasmodium yoelii parasites and human-infectious Plasmodium falciparum parasites. The data robustly define mRNAs and proteins that are upregulated in oocyst sporozoites (UO ...[more]

PMID: 31673027

Similar Datasets

Project description:For malaria transmission, the parasite must undergo sexual differentiation into mature gametocytes. However, the molecular basis for this critical transition in the parasites life cycle is unknown. Six previously uncharacterized genes, Pfg14.744, Pfg14.745, Pfg14.748, Pfg14.763, Pfg14.752 and Pfg6.6 that are members of a 36 gene Plasmodium falciparum-specific subtelomeric superfamily were found to be expressed in parasites that are committed to sexual development as suggested by co-expression of Pfs16 and Pfg27. Northern blots demonstrated that Pfg14.744 and Pfg14.748 were first expressed before the parasites differentiated into morphologically distinct gametocytes, transcription continued to increase until stage II gametocytes were formed and then rapidly decreased. Immunofluorescence assays indicated that both proteins were only produced in the subpopulation of ring stage parasites that are committed to gametocytogenesis and both localized to the parasitophorous vacuole (PV)b of the early ring stage parasites. As the parasites continued to develop Pfg14.748 remained within the parasitophorous vacuole, while Pfg14.744 was detected in the erythrocyte. The 5' flanking region of either gene alone was sufficient to drive early gametocyte specific expression of green fluorescent protein (GFP). In parasites transfected with a plasmid containing the Pfg14.748 5' flanking region immediately upstream of GFP, fluorescence was observed in a small number of schizonts the cycle before stage I gametocytes were observed. This expression pattern is consistent with commitment to sexual differentiation prior to merozoite release and erythrocyte invasion. Further investigation into the role of these genes in the transition from asexual to sexual differentiation could provide new strategies to block malaria transmission. Microarray analysis was used to compare two clones derived from Plasmodium falciparum strain 3D7 parasites that differ in their ability to undergo gametocytogenesis. Clone G+ produces gametocytes and clone G- produces very few if any gametocytes. RNA was harvested from the cultures when the asexual parasitemia was 0.9-1.48% (day 4) (n=4) after setting up the gametocyte cultures and 5.2-5.58% (day 6) (n=4) prior to the appearance of morphologically distinct gametocytes and used to generate cDNA that was labeled with Cy3 or Cy5 and hybridized to the Plasmodium falciparum 70 mer oligonucleotide microarray developed by DeRisi and co-workers.