Proteomics

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Porphyromonas gingivalis Outer Membrane Vesicle and cell Proteome in heme excess and heme limited


ABSTRACT: Porphyromonas gingivalis is an anaerobic, Gram-negative oral pathogen associated with chronic periodontitis. P. gingivalis has an obligate requirement for heme which it obtains from the host. Heme availability has been linked to disease initiation and progression. In this study we used continuous culture of the bacterium to determine the effect of heme limitation and excess on the P. gingivalis proteome. Four biological replicates of whole cell lysate (WCL) and outer membrane vesicle (OMV) samples were digested with trypsin and analysed by tandem mass spectrometry and MaxQuant label-free quantification. In total, 1211 proteins were quantified with 108 and 49 proteins significantly changing in abundance more than 1.5-fold (p<0.05) in the WCLs and OMVs respectively. The proteins most upregulated in response to heme limitation were those involved in binding and transporting heme while the four proteins most upregulated in the heme excess condition constitute a putative heme efflux system. In general, the protein abundance ratios obtained for OMVs and WCLs agreed, indicating that changes to the OM protein composition are passed on to OMVs, however 16 proteins were preferentially packaged into OMVs in one condition more than the other. In particular, moonlighting cytoplasmic proteins were preferentially associated with OMVs under heme excess.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Porphyromonas Gingivalis W50

SUBMITTER: Paul Veith  

LAB HEAD: Eric Charles Reynolds

PROVIDER: PXD009730 | Pride | 2018-05-18

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
160822_PaulV_1_1.raw Raw
160822_PaulV_1_2.raw Raw
160822_PaulV_1_3.raw Raw
160822_PaulV_2_1.raw Raw
160822_PaulV_2_2.raw Raw
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Publications

Outer Membrane Vesicle Proteome of Porphyromonas gingivalis Is Differentially Modulated Relative to the Outer Membrane in Response to Heme Availability.

Veith Paul D PD   Luong Caroline C   Tan Kheng H KH   Dashper Stuart G SG   Reynolds Eric C EC  

Journal of proteome research 20180529 7


Porphyromonas gingivalis is an anaerobic, Gram-negative oral pathogen associated with chronic periodontitis. P. gingivalis has an obligate requirement for heme, which it obtains from the host. Heme availability has been linked to disease initiation and progression. In this study we used continuous culture of the bacterium to determine the effect of heme limitation and excess on the P. gingivalis proteome. Four biological replicates of whole cell lysate (WCL) and outer membrane vesicle (OMV) samp  ...[more]

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