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Aluminum and salt induced protein changes in tomato plants transformed with bar gene

ABSTRACT: In this project, seedlings of tomato (Solanum lycopersicum) ‘Money Maker’ plants transformed with a bar gene were treated under 20 µM Al3+ and salt (200 mM NaCl) for two weeks. The treatments were conducted in hydroponic solutions in a greenhouse at 24±2C without supplemental light. Roots harvested were immediately frozen in liquid nitrogen and stored at -80 oC. The treatment experiment was conducted at Agricultural Research Station, Tennessee State University, Nashville, TN 37209

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Plant Cell, Root

SUBMITTER: Ted Thannhauser

LAB HEAD: Theodore Thannhauser

PROVIDER: PXD009848 | Pride | 2020-04-09

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	25Dec2017_Suping_XSexp3_ITAG3.2.mgf	Mgf
	25Dec2017_Suping_XSexp3_ITAG3.2.msf	Msf
	25Dec2017_Suping_XSexp3_ITAG3.2.mzML	Mzml
	25Dec2017_Suping_XSexp3_ITAG3.2.mzid	Mzid
	28Dec2017_Suping_XSexp3_F1.raw	Raw

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1 - 5 of 16

Publications

Al-induced proteomics changes in tomato plants over-expressing a glyoxalase I gene.

Sun Xudong X Li Hui H Thapa Santosh S Reddy Sangireddy Sasikiran S Pei Xiaobo X Liu Wei W Jiang Yuping Y Yang Shaolan S Hui Dafeng D Bhatti Sarabjit S Zhou Suping S Yang Yong Y Fish Tara T Thannhauser Theodore W TW

Horticulture research 20200401 1

Glyoxalase I (Gly I) is the first enzyme in the glutathionine-dependent glyoxalase pathway for detoxification of methylglyoxal (MG) under stress conditions. Transgenic tomato 'Money Maker' plants overexpressing tomato <i>SlGlyI</i> gene (tomato unigene accession SGN-U582631/Solyc09g082120.3.1) were generated and homozygous lines were obtained after four generations of self-pollination. In this study, <i>SlGlyI-</i>overepxressing line (GlyI), wild type (WT, negative control) and plants transforme ...[more]

PMID: 32257229

Similar Datasets

Aluminum and salt induced protein changes in tomato overexpressing a glyoxalase I gene

Project description:In this project, seedlings of tomato (Solanum lycopersicum) ‘Money Maker’ plants overexpressing a glyoxalase I gene were treated under 20 µM Al3+ and salt (200 mM NaCl) for two weeks. The treatments were conducted in hydroponic solutions in a greenhouse at 24±2C without supplemental light. Roots harvested were immediately frozen in liquid nitrogen and stored at -80 oC. The treatment experiment was conducted at Agricultural Research Station, Tennessee State University, Nashville, TN 37209

2020-04-09 | PXD009456 | Pride

(TMT)-quantitative proteomic study of Bacillus cereus tsu1

Project description:In this project, we report the bacterial proteome expression profile change for different time points when culturing in rapeseed cake substrate (RCS). Bacillus cereus tsu1 was cultured in RCS (25g/L) for 12 hours, 24 hours and 48 hours. At each time point, cells were stained with Sudan Black to monitor PHB accumulation. Bacterial cells were loaded with PHB after 12-hour culture and significant degradation of PHB was observed after 48-hour culture. Bacterial proteome profile changes were identified using tandem mass tags mass spectrometry (TMT-MS)-based quantitative proteomics analysis. From TMT proteomics analysis, 3,237 proteins were detected and quantified from the bacterial pellet protein extraction, from which proteins related with PHB biosynthesis and degradation were identified. Quantitative analysis revealed that 146 (between 12h and 24h samples), and 158 (between 12h and 48h) proteins showed significant differences. Several STRING protein interaction networks were developed for significantly changed protein related with sporulation and anaerobic respiration.

2021-09-08 | PXD009960 | Pride

Association of proteomics changes with Al-sensitive root zones in switchgrass, part 2

Project description:In this project, we report on aluminum (Al)-induced root proteomic changes in switchgrass. After growth in a hydroponic culture system supplemented with 400μM of Al, plants began to show signs of physiological stress such as a reduction in photosynthetic rate. At this time, the basal 2‐cm long root tips were harvested and divided into two segments, each of 1‐cm in length, for protein extraction. Al-induced changes in proteomes were identified using tandem mass tags mass spectrometry (TMT-MS)-based quantitative proteomics analysis. A total of 216 proteins (approximately 3.6% of total proteins) showed significant differences between non-Al treated control and treated groups with significant fold change (twice the standard deviation; FDR adjusted p-value, q<0.05). The basal root tip tissues expressed more dramatic proteome changes (164 significantly changed proteins; 3.9% of total proteins quantified) compared to the elongation/maturation zones (52 significantly changed proteins, 1.1% of total proteins quantified). Significantly changed proteins from the basal-1cm root apex tissues were clustered into 25 biological pathways, three proteins in cell cycles were all at a reduced abundance level compared to the non-treated control group. In the root elongation and maturation zone tissues, the identified proteins were placed into 18 pathways, proteins in secondary cell wall formation (lignin biosynthesis) were identified. Several STRING protein interaction networks were developed for these Al-induced significantly changed proteins. This study has identified a large number of Al-responsive proteins, including transcription factors, which will be used for exploring new Al tolerance genes and mechanisms.

2018-03-19 | PXD009125 | Pride

Mouse brain LC-MS - KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a

Project description:To investigate the molecular mechanisms by which KIBRA regulates exosome secretion, we performed mass spectral (MS) analysis and an isobaric tag for relative and absolute quantitation (iTRAQ) assay to screen the differentially-expressed proteins in the brains of KIBRA-KO mice compared with their WT littermates.

2019-04-02 | PXD012430 | Pride

Association of proteomics changes with Al-sensitive root zones in switchgrass

2018-03-23 | PXD008882 | Pride

Quantitative proteomics confirms CpKRS is over-expressed in genetically modified strain.

Project description:C. parvum was genetically modified to over-express CpKRS. The CpTK locus was replaced wit a cassette containing CpKRS and the dual reporter-selection marker, Nluc-NeoR, driven by the CpEnolase promoter. Quantitative proteomics analysis confirms over-expression of CpKRS and similar significantly increased expression of Nluc-NeoR.

2024-07-05 | PXD050089 | Pride

Human bladder cancer LC MS/MS - A Comprehensive Investigation toward the Indicative Proteins of Bladder Cancer in Urine: From Surveying Cell Secretomes to Verifying Urine Proteins

Project description:Urine is an ideal material to study and examine the bladder cancer (BCa) biomarkers, whereas exploration to the corresponding protein candidates is confronting technique challenges. Herein, we proposed a comprehensive strategy of searching the urine proteins related to BCa. The strategy consists of three core combinations, screening the candidates in the secreted proteins derived from the BCa cell lines and verifying them in the patient urines, defining the differential proteins through two-dimensional electrophoresis (2DE) and isobaric tags for relative and absolute quantitation (iTRAQ), and implementing quantitative analysis in profiling and targeting proteomics. The differential proteins were globally and quantitatively determined between the two typical cell lines of BCa, T24 and 5637, and the immortalized normal uroepithelium cell line, SV-HUC-1, while the BCa related proteins were verified between the relatively normal and patient urines. With proteomic survey combined with 2DE and iTRAQ, a total of 724 secreted proteins were identified as the BCa related proteins. With label-free quantitative proteomics, the 96 candidates were detected in the pooled urine samples. Furthermore, the multiple reaction monitoring (MRM)-based quantification was adapted to verify these urine proteins in individual urines that were collected from 23 BCa patients and 24 relative normal people, and resulted in the 10 urine proteins with significant abundance differences between the two groups. Of the potential indicators for BCa, analysis of receiver operating characteristics (ROC) revealed the combination of complement component 3 (CO3) and lactose dehydrogenase B (LDHB) was more sensitive and efficient to distinguish healthy and disease urine. The discovery of the indicative proteins of BCa through our strategy has thus paved an avenue to further validation of BCa biomarkers in urine.

2016-06-17 | PXD003557 | Pride

LCM-quantitative proteomics analysis of Al-sensitive tomato root cells

Project description:Aluminum toxicity in acid soil is a major stress to plant growth. The primary target is in root-tips where the epidermal and outer cortex layers of cells in the root transition zone area are more sensitive to the toxic ions. This project used laser capture microdissection (LCM) technology to isolate these Al-sensitive cells. Tandem mass tag (TMT) proteomics was used to identify Al- induced proteomes. The information was used to determine functions of protein changes in response to Al toxicity in these Al-sensitive root cells.

2019-11-07 | PXD010459 | Pride

The liver,spleen,brain,intestine and kidney tissue of the Oreochromis niloticus which infected with Streptococcus agalactiae LC-MSMS

Project description:The Oreochromis niloticus was infected with S.agalactiae strain YM001, than collected the liver,spleen,brain,intestine and kidney tissue for proteome

2020-05-26 | PXD014616 | Pride

Heat-induced proteomes in tomato pollen mother cells

Project description:Tomato pollen production and viability is highly vulnerable to higher temperature. Hot summers with temperature reaching above 32°C can disrupt production of viable pollens and fruit set, resulting in yield loss. In recent years, temperature above 35-38oC has become a norm during mid-summer with potential adverse impacts on the production of tomatoes and many other crop species. Pollens are developed through the microsporogenesis and micro-gametogenesis stages. The most heat sensitive period is from the meiotic process of the microsporocytes, at the young microspore stage (uninucleate stage of microspore) to during late pollen development (pollen mitosis). This project studied the heat-induced proteomes in microsporocyte, also called pollen mother cells (PMC). Homogenous PMC samples were collected from cross-sectioned frozen fresh anther tissues of tomato ‘Maxifort’ using laser capture microdissection (LCM). Tandem mass tag (TMT) proteomics analysis was conducted to identify proteomics changes related to heat tolerance during pollen development.

2022-04-07 | PXD018915 | Pride

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