Dataset Information

Profiling the proteomic inflammatory state of human astrocytes using DIA mass spectrometry

ABSTRACT: In the present study we used DIA MS to characterize the profile of over 4,000 proteins in immortalized human astrocytes exposed to TNF, IL-1β, and LPS, as well as in primary human astrocytes cocultured with brain endothelial cells and exposed to LPS. For both immortalized and primary astrocytes, DIA MS data were matched against a spectral library generated by merging DDA MS runs from both unfractionated and fractionated cell lysates.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Astrocyte

SUBMITTER: Vito Dozio

LAB HEAD: Jean-Charles Sanchez

PROVIDER: PXD009891 | Pride | 2018-12-10

REPOSITORIES: Pride

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			Action	DRS
	20170918_LU50_Immortalized_DDA_OGE10_R1.raw	Raw
	20170918_LU50_Immortalized_DDA_OGE10_R2.raw	Raw
	20170918_LU50_Immortalized_DDA_OGE11_R1.raw	Raw
	20170918_LU50_Immortalized_DDA_OGE11_R2.raw	Raw
	20170918_LU50_Immortalized_DDA_OGE12_R1.raw	Raw

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Profiling the proteomic inflammatory state of human astrocytes using DIA mass spectrometry.

Dozio Vito V Sanchez Jean-Charles JC

Journal of neuroinflammation 20181130 1

<h4>Background</h4>Astrocytes are the most abundant cells in the central nervous system and are responsible for a wide range of functions critical to normal neuronal development, synapse formation, blood-brain barrier regulation, and brain homeostasis. They are also actively involved in initiating and perpetuating neuroinflammatory responses. However, information about their proteomic phenotypes under inflammation is currently limited.<h4>Method</h4>Data-independent acquisition mass spectrometry ...[more]

PMID: 30501627

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Project description:Research regarding the role of astrocytes as M-NM-2-amyloid (AM-NM-2) degrading cells has broadened our view about the mechanisms how these common glia cells function in AlzheimerM-bM-^@M-^Ys disease (AD). Based on previous studies adult mouse astrocytes are able to degrade AM-NM-2 deposits from AD mouse model and human brain tissue sections ex vivo, contrary to neonatal astrocytes. In this study, we studied the possible altered gene expression profiles of adult and neonatal astrocytes cultured for 22 h on top of the AM-NM-2 burdened tg APdE9 or wild-type mouse brain sections using whole genome microarrays. 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