Human THP1 macrophages after interacting with C. albicans, using ATP-binding protein enrichment strategy

ABSTRACT: Macrophages are involved in the primary human host response to pathogens such as Candida albicans. After pathogen recognition by specific receptors, a cascade of signaling pathways is activated, leading to the production of cytokines, chemokines and antimicrobial peptides. ATP binding proteins are crucial for the regulation of signaling pathways. Taking into account the fact that proteomics can provide important information regarding these molecular mechanisms, a quantitative proteomic approach was carried out for the study of human macrophage ATP-binding proteins after interaction with C. albicans. A total of 547 non-redundant proteins were quantified in at least two biological replicates and with a standard deviation lower than 0.3. Among them, 59 proteins were considered differentially abundant during macrophage interaction with C. albicans. Moreover, with the proteins enriched in ATP-binding proteins a phosphopeptide enrichment was performed, and a total of 70 phosphosites was quantified, using the same filtering criteria. We repeated the search of the .raw files in the new nexprot database (NextProt v2.15.0, 20230 protein entries) that we also uploaded in this project. In summary, in this project we uploaded: a. Quantitative proteomic results- .raw files; .msf files; mgf and excel table with quantitative results b. Quantitative phosphor results - .raw files; .msf files; mgf and excel table with quantitative results c. Identification results with recent version of Nexprot-mzIdentML.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Candida Albicans (yeast)

TISSUE(S): Cell Culture

SUBMITTER: Concha Gil  

LAB HEAD: Concha Gil Garcia

PROVIDER: PXD009938 | Pride | 2019-04-18

REPOSITORIES: Pride