Dataset Information

Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography-Tandem Mass Spectrometry

ABSTRACT: We performed proteomic analyses of human olfactory epithelial tissue to identify missing proteins using liquid chromatography-tandem mass spectrometry. Using a next-generation proteomic pipeline with a <1.0% false discovery rate at the peptide and protein levels, we identified 3,731 proteins, among which five were missing proteins (P0C7M7, P46721, P59826, Q658L1, and Q8N434). We validated the identified missing proteins using the corresponding synthetic peptides. We also identified 49 and 50 alternative splicing variants mapped at the neXtProt and GENCODE databases, respectively, and 2,000 additional single amino acid variants.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Nasal Epithelium

SUBMITTER: Ji Eun Jeong

LAB HEAD: Jong Shin Yoo

PROVIDER: PXD010025 | Pride | 2018-08-17

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	20170620_50cmColum_HHY_Whole_Ingel_Fraction_01.raw	Raw
	20170620_50cmColum_HHY_Whole_Ingel_Fraction_02.raw	Raw
	20170620_50cmColum_HHY_Whole_Ingel_Fraction_03.raw	Raw
	20170620_50cmColum_HHY_Whole_Ingel_Fraction_04.raw	Raw
	20170620_50cmColum_HHY_Whole_Ingel_Fraction_05.raw	Raw

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Publications

Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography-Tandem Mass Spectrometry.

Hwang Heeyoun H Jeong Ji Eun JE Lee Hyun Kyoung HK Yun Ki Na KN An Hyun Joo HJ Lee Bonghee B Paik Young-Ki YK Jeong Tae Seok TS Yee Gi Taek GT Kim Jin Young JY Yoo Jong Shin JS

Journal of proteome research 20180820 12

We performed proteomic analyses of human olfactory epithelial tissue to identify missing proteins using liquid chromatography-tandem mass spectrometry. Using a next-generation proteomic pipeline with a < 1.0% false discovery rate at the peptide and protein levels, we identified 3731 proteins, among which five were missing proteins (P0C7M7, P46721, P59826, Q658L1, and Q8N434). We validated the identified missing proteins using the corresponding synthetic peptides. No olfactory receptor (OR) prote ...[more]

PMID: 30113170

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