Dataset Information

Forward and reverse degradomics defines the proteolytic landscape of human knee osteoarthritic cartilage and the role of the serine protease HtrA1

ABSTRACT: Proteolytic destruction of articular cartilage is a major pathogenic mechanism in osteoarthritis (OA), but was not previously investigated on a proteome-wide scale. We sought to define the human knee OA cartilage degradome and the contribution therein of the serine protease HtrA1, using an N-terminomics strategy, terminal amine isotopic labeling of substrates (TAILS).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cartilage

SUBMITTER: Sumit Bhutada

LAB HEAD: Suneel Apte

PROVIDER: PXD028320 | Pride | 2023-11-03

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	HtrA1_Talus_TAILS.mzML	Mzml
	HtrA1_Talus_TAILS.mzid.gz	Mzid
	HtrA1_Talus_TAILS1-Fr1.raw	Raw
	HtrA1_Talus_TAILS1-Fr2.raw	Raw
	HtrA1_Talus_TAILS1-Fr3.raw	Raw

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Forward and reverse degradomics defines the proteolytic landscape of human knee osteoarthritic cartilage and the role of the serine protease HtrA1.

Bhutada S S Li L L Willard B B Muschler G G Piuzzi N N Apte S S SS

Osteoarthritis and cartilage 20220324 8

<h4>Objectives</h4>Proteolytic destruction of articular cartilage, a major pathogenic mechanism in osteoarthritis (OA), was not previously investigated by terminomics strategies. We defined the degradome of human knee OA cartilage and the contribution therein of the protease HtrA1 using Terminal Amine Isotopic Labeling of Substrates (TAILS).<h4>Design</h4>Proteins from OA cartilage taken at knee arthroplasty (n = 6) or separately, from healthy cartilage incubated in triplicate with/without activ ...[more]

PMID: 35339693

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