Proteomics

Dataset Information

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Dynamic variation in ejaculate expenditure and seminal fluid proteome over a mating sequence: the signature of female novelty and male social status in red junglefowl


ABSTRACT: Seminal fluid sample were collected from dominant and subdominant males who were re peatedly mated to first female followed by a mating to the novel second female. The goal in to monitor seminal fluid investment and composition changed dynamically over the successive matings. Our results indicate that ejaculate expenditure over a mating sequence is associated with dynamic SFP changes driven by a combination of depletion and strategic allocation.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Semen

SUBMITTER: Kirill Borziak  

LAB HEAD: Steve Dorus

PROVIDER: PXD010214 | Pride | 2019-11-14

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
P093_6216_Wa1sG1_L1_1.raw Raw
P093_6216_Wa2sG1_L2_1.raw Raw
P093_6216_Wa3sG1_L3_1.raw Raw
P093_6216_Wa4sG1_L4_1.raw Raw
P093_6216_Wa6sG2_L5_1.raw Raw
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Publications

Female novelty and male status dynamically modulate ejaculate expenditure and seminal fluid proteome over successive matings in red junglefowl.

Alvarez-Fernandez Aitor A   Borziak Kirill K   McDonald Grant C GC   Dorus Steve S   Pizzari Tommaso T  

Scientific reports 20190410 1


Theory predicts that males will strategically invest in ejaculates according to the value of mating opportunities. While strategic sperm allocation has been studied extensively, little is known about concomitant changes in seminal fluid (SF) and its molecular composition, despite increasing evidence that SF proteins (SFPs) are fundamental in fertility and sperm competition. Here, we show that in male red junglefowl, Gallus gallus, along with changes in sperm numbers and SF investment, SF composi  ...[more]

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