Proteomics

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Infection by wMel Wolbachia alters female post-mating behaviors and physiology in the dengue vector mosquito Aedes aegypti


ABSTRACT: Globally invasive Aedes aegypti mosquitoes disseminate numerous arboviruses that impact human health. One promising method to control Ae. aegypti populations is transinfection with the intracellular bacterium Wolbachia pipientis, a symbiont that naturally infects ~40-52% of insects but is normally absent from Ae. aegypti. Transinfection of Ae. aegypti with the wMel Wolbachia strain induces cytoplasmic incompatibility (CI), allowing infected individuals to rapidly invade native populations. Further, wMel Wolbachia-infected females display refractoriness to medically relevant arboviruses. Thus, wMel Wolbachia-infected Ae. aegypti are being released in several areas to replace native populations, thereby suppressing disease transmission by this species. Wolbachia is reported to have minimal effects on Ae. aegypti fertility, but its influence on other reproductive processes is unknown. Female insects undergo several post-mating physiological and behavioral changes required for optimal fertility. Post-mating responses (PMRs) in female insects are typically elicited by receipt of male seminal fluid proteins (SFPs) transferred with sperm during mating, but can be modified by other factors, such as adult age, nutritional status, and microbiome composition. To assess how Wolbachia infection influences Ae. aegypti female PMRs, we collected wMel Wolbachia-infected Ae. aegypti from the field in Medellín, Colombia and introduced the bacterium into our laboratory strain. We found that Wolbachia influences female fecundity, fertility, and re-mating incidence. Further, we observed that Wolbachia significantly extends longevity of virgin females. Changes in female PMRs are not due to defects in sperm transfer by infected males, or sperm storage by infected females. Using proteomic methods to examine the seminal proteome of infected males, we found that Wolbachia infection has a moderate effect on SFP composition. However, we identified 125 Wolbachia proteins that are paternally transferred to females by infected males. Surprisingly, the CI factor proteins (Cifs), were not detected in the ejaculates of Wolbachia-infected males. Our findings indicate that Wolbachia infection of Ae. aegypti alters female post-mating responses, potentially influencing control programs that utilize Wolbachia-infected individuals.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Aedes Aegypti Aegypti

TISSUE(S): Bursa Copulatrix

SUBMITTER: steve dorus  

LAB HEAD: Steve Dorus

PROVIDER: PXD043965 | Pride | 2023-10-24

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
P1179_Gel1_V1_1.mgf Mgf
P1179_Gel1_V1_1.raw Raw
P1179_Gel1_V1_2.mgf Mgf
P1179_Gel1_V1_2.raw Raw
P1179_Gel1_V1_3.mgf Mgf
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Publications

wMel Wolbachia alters female post-mating behaviors and physiology in the dengue vector mosquito Aedes aegypti.

Osorio Jessica J   Villa-Arias Sara S   Camargo Carolina C   Ramírez-Sánchez Luis Felipe LF   Barrientos Luisa María LM   Bedoya Carolina C   Rúa-Uribe Guillermo G   Dorus Steve S   Alfonso-Parra Catalina C   Avila Frank W FW  

Communications biology 20230821 1


Globally invasive Aedes aegypti disseminate numerous arboviruses that impact human health. One promising method to control Ae. aegypti populations is transinfection with Wolbachia pipientis, which naturally infects ~40-52% of insects but not Ae. aegypti. Transinfection of Ae. aegypti with the wMel Wolbachia strain induces cytoplasmic incompatibility (CI), allows infected individuals to invade native populations, and inhibits transmission of medically relevant arboviruses by females. Female insec  ...[more]

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