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Identification of ZNF217 interactors in MCF7 breast cancer cell line

ABSTRACT: Identification of TRPS1 interactors in MCF7 breast cancer cell line.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Aurelien Serandour

LAB HEAD: Jason Carroll

PROVIDER: PXD010312 | Pride | 2018-07-17

REPOSITORIES: Pride

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			Action	DRS
	PR189_AUS_120412_JP289-01.msf	Msf
	PR189_AUS_120412_JP289-02.msf	Msf
	PR189_AUS_120412_JP289-03.msf	Msf
	PR189_AUS_120412_JP289-04.msf	Msf
	PR189_AUS_120412_JP289-05.msf	Msf

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Publications

TRPS1 regulates oestrogen receptor binding and histone acetylation at enhancers.

Serandour A A AA Mohammed H H Miremadi A A Mulder K W KW Carroll J S JS

Oncogene 20180612 39

The chromatin state is finely tuned to regulate function and specificity for transcription factors such as oestrogen receptor alpha (ER), which contributes to cell growth in breast cancer. ER transcriptional potential is mediated, in large part, by the specific associated proteins and co-factors that interact with it. Despite the identification and characterisation of several ER coregulators, a complete and systematic view of ER-regulating chromatin modifiers is lacking. By exploiting a focused ...[more]

PMID: 29895970

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Project description:Wild type (wt) MCF7 cells, modelling breast cancer at primary diagnosis, were cultured in phenol red-free RPMI supplemented with 10% FBS and 1nM estradiol (E2). Long-term oestrogen deprived (LTED) cell lines, which model resistance to endocrine therapy, were cultured in phenol red-free RPMI in the absence of exogenous E2 and supplemented with 10% dextran charcoal-stripped bovine serum (DCC). Samples were harvested at baseline and at the point of resistance (LTED). In order to do comparative analysis in the ER-interactome of wt-MCF7 and MCF7-LTED cells, ER-RIME (rapid immunoprecipitation mass spectrometry of endogenous proteins) was conducted in these cells.

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RIME against PBX1 protein in P19.6 mouse embryonal carcinoma cells treated for 48 hours with all-trans retinoic acid

Project description:Identification of PBX1 interactors in P19.6 mouse embryonal carcinoma cells treated for 48 hours with all-trans retinoic acid.

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ChIPseq of DDX3X, DDX54, ERalpha and H3K4me3 in human breast cancer MCF7 cells

Project description:We aimed to investigate the chromatin binding activity of DDX3X and DDX54 RNA helicases in human ER -dependent breast cancer MCF7 cells. We run a parallel chromatin binding profiling of ER ChIP-seq. H3K4me3 profiling was used as a quality control of the ChIP-seq procedure.

2019-11-14 | E-MTAB-6211 | biostudies-arrayexpress

HAX1-interactome in breast cancer cell line (MCF7)

Project description:The MCF7 cell line represents a typical epithelial cell line and corresponds to luminal A breast cancer (estrogen-responsive). Overexpression of HAX1 was demonstrated in MCF7 cell line as well as in breast cancer samples, suggesting a role of HAX1 in breast cancer progression. HAX1 is a 32-kDa protein of unknown structure, involved in the regulation of apoptosis, cell migration and calcium homeostasis. It was also shown to bind mRNA. Scarcity of structural elements and the presence of a disordered region, inferred from HAX1 sequence, suggests that HAX1 is intrinsically disordered, and may have many protein-protein interactions. So far about 40 different proteins were characterized as HAX1 protein partners. In the present work, applying immunoaffinity chromatography coupled with mass spectrometry, we identified new candidates for HAX1 binding partners in breast cancer cells. Newly identified proteins may be divided into three, partially overlapping groups: cytoskeleton-associated proteins, GTP-ase associated proteins and RNA-binding proteins. These results imply that HAX1 has more protein partners than hitherto described. Subsequent analysis of these interactions may shed some light into molecular mechanisms of HAX1 functions.

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Expression profiles of MCF7-CV and MCF7-6SA

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Chromatin immunopreciptiation to determine RNA polymerase II binding in human cell line MCF7 treated with estradiol

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2008-06-09 | E-MEXP-1644 | biostudies-arrayexpress

Effect of BORIS depletion on transcriptome of breast cancer cell line MCF7

Project description:Transcriptome analysis of BORIS depleted MCF7 cells To delineate the alternative pre-mRNA splicing program mediated by BORIS in breast cancer cells, BORIS was depleted in MCF7 using shRNA, and the resultant changes in alternative pre-mRNA splicing and mRNA expression pattern was profiled using Affymetrix Human Transcriptome 2.0 Array

2017-09-25 | GSE81001 | GEO

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