Proteomics

Dataset Information

0

HAX1-interactome in breast cancer cell line (MCF7)

ABSTRACT: The MCF7 cell line represents a typical epithelial cell line and corresponds to luminal A breast cancer (estrogen-responsive). Overexpression of HAX1 was demonstrated in MCF7 cell line as well as in breast cancer samples, suggesting a role of HAX1 in breast cancer progression. HAX1 is a 32-kDa protein of unknown structure, involved in the regulation of apoptosis, cell migration and calcium homeostasis. It was also shown to bind mRNA. Scarcity of structural elements and the presence of a disordered region, inferred from HAX1 sequence, suggests that HAX1 is intrinsically disordered, and may have many protein-protein interactions. So far about 40 different proteins were characterized as HAX1 protein partners. In the present work, applying immunoaffinity chromatography coupled with mass spectrometry, we identified new candidates for HAX1 binding partners in breast cancer cells. Newly identified proteins may be divided into three, partially overlapping groups: cytoskeleton-associated proteins, GTP-ase associated proteins and RNA-binding proteins. These results imply that HAX1 has more protein partners than hitherto described. Subsequent analysis of these interactions may shed some light into molecular mechanisms of HAX1 functions.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Mcf-7 Cell

SUBMITTER: Tymon Rubel  

LAB HEAD: Ewa Grzybowska

PROVIDER: PXD007888 | Pride | 2019-11-14

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
70630_grzy_M_HAX_1.dat-pride.pride.mgf.gz Mgf
70630_grzy_M_HAX_1.dat-pride.pride.mztab.gz Mztab
70630_grzy_M_HAX_1.dat-pride.xml.gz Xml
70630_grzy_M_HAX_1.dat.gz Other
70630_grzy_M_HAX_1.raw.gz Raw
Items per page:
1 - 5 of 30
altmetric image

Publications

The interactome of multifunctional HAX1 protein suggests its role in the regulation of energy metabolism, de-aggregation, cytoskeleton organization and RNA-processing.

Wakula Maciej M   Balcerak Anna A   Rubel Tymon T   Chmielarczyk Mateusz M   Konopinski Ryszard R   Lyczek Filip F   Grzybowska Ewa A EA  

Bioscience reports 20201101 11

HCLS1-associated protein X-1 (HAX1) is a multifunctional protein involved in many cellular processes, including apoptosis, cell migration and calcium homeostasis, but its mode of action still remains obscure. Multiple HAX1 protein partners have been identified, but they are involved in many distinct pathways, form different complexes and do not constitute a coherent group. By characterizing HAX1 protein interactome using targeted approach, we attempt to explain HAX1 multiple functions and its ro  ...[more]

PMID: 33146709

Publication: 1/2

Similar Datasets

Proteomics HAX1-interactome in cervical cancer cell line (HeLa)
2020-11-11 | PXD007887 | Pride
Genomics ChIPseq of DDX3X, DDX54, ERalpha and H3K4me3 in human breast cancer MCF7 cells
2019-11-14 | E-MTAB-6211 | biostudies-arrayexpress
Proteomics Identification of AKTIP protein interaction partners in breast cancer cells MCF7
2023-01-02 | PXD035716 | Pride
Proteomics Identification of ZNF217 interactors in MCF7 breast cancer cell line
2018-07-17 | PXD010312 | Pride
Proteomics RIME against TRPS1 in breast cancer cell lines
2018-06-12 | PXD009863 | Pride
Proteomics A Multi-Faceted Analysis Showing CRNDE Transcripts and a Recently Confirmed Micropeptide as Important Players in Ovarian Carcinogenesis (CRNDEP co-immunoprecipitation)
2024-04-28 | PXD044160 | Pride
Proteomics 2D-PAGE coupled with nLC-MS/MS-based identification of differentialy expressed proteins and tumorigenic pathways in MCF7 breast cancer cell line transfected for JTB protein silencing
2024-01-26 | PXD046265 | Pride
Proteomics MS-based characterization of YTHDC1 interactome
2023-01-11 | PXD037414 | Pride
Proteomics Nuclear Estrogen Receptor alpha (ERα) interactome following estrogen and antiestrogen treatment.
2020-09-02 | PXD018709 | Pride
Proteomics Catechol-O-methyl transferase suppresses cell invasion and interplays with MET signaling in estrogen dependent breast cancer
2023-01-31 | PXD033833 | Pride