Dataset Information

Human hTERT-RPE1 cell AP-MS Dynein-2 intermediate chains

ABSTRACT: Affinity isolation of proteins interacting with dynein-2 intermediate chains WDR34 and WDR6 in both WT and WDR34/WDR60 knockout cell lines0

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

SUBMITTER: David Stephens

LAB HEAD: David Stephens

PROVIDER: PXD010398 | Pride | 2018-11-04

REPOSITORIES: Pride

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Publications

Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function.

Vuolo Laura L Stevenson Nicola L NL Heesom Kate J KJ Stephens David J DJ

eLife 20181016

The dynein-2 microtubule motor is the retrograde motor for intraflagellar transport. Mutations in dynein-2 components cause skeletal ciliopathies, notably Jeune syndrome. Dynein-2 contains a heterodimer of two non-identical intermediate chains, WDR34 and WDR60. Here, we use knockout cell lines to demonstrate that each intermediate chain has a distinct role in cilium function. Using quantitative proteomics, we show that WDR34 KO cells can assemble a dynein-2 motor complex that binds IFT proteins ...[more]

PMID: 30320547

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