Dataset Information

Protein interactions with HA-tagged cytoplasmic dynein-2 DATASET1

ABSTRACT: To identify those proteins interacting with the cytoplasmic dynein-2 using mammalian cell lines stably expressing HA-tagged intermediate chain subunits, WDR34 (DYNC2I2) or WDR60 (DYNC2I1).

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

SUBMITTER: David Stephens

LAB HEAD: David Stephens

PROVIDER: PXD031151 | Pride | 2023-01-24

REPOSITORIES: Pride

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Multiple interactions of the dynein-2 complex with the IFT-B complex are required for effective intraflagellar transport.

Hiyamizu Shunya S Qiu Hantian H Vuolo Laura L Stevenson Nicola L NL Shak Caroline C Heesom Kate J KJ Hamada Yuki Y Tsurumi Yuta Y Chiba Shuhei S Katoh Yohei Y Stephens David J DJ Nakayama Kazuhisa K

Journal of cell science 20230207 5

The dynein-2 complex must be transported anterogradely within cilia to then drive retrograde trafficking of the intraflagellar transport (IFT) machinery containing IFT-A and IFT-B complexes. Here, we screened for potential interactions between the dynein-2 and IFT-B complexes and found multiple interactions among the dynein-2 and IFT-B subunits. In particular, WDR60 (also known as DYNC2I1) and the DYNC2H1-DYNC2LI1 dimer from dynein-2, and IFT54 (also known as TRAF3IP1) and IFT57 from IFT-B contr ...[more]

PMID: 36632779

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	Laura_Vuolo_171017_5Plex_TMT.pdResult	Other