Proteomics

Dataset Information

0

Sialo-glycoproteome of Human lung cancer cell by LC-MSMS

ABSTRACT: Alterations in protein glycosylation, especially the terminal sialylation, are closely correlated with physiological and pathological regulation. Due to the low ionization efficiency of sialo-glycopeptides and frequently observed dissociation of sialic acid residues during mass spectrometry analysis, tools to enrich the sialo-glycopeptides are essential to enhance detection sensitivity and identification efficiency in sialo-glycoproteomics. In this study, we present the first application of zwitterionic hydrophilic interaction chromatography (ZIC-cHILIC) material in StageTip for simultaneous enrichment and fractionation of intact glycopeptides at proteome scale. With the demonstrated enrichment specificity and identification depth, the stepwise-ZIC-cHILIC can be an efficient enrichment method for the discovery of glycosylation sites and native glycotope for many sample types.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Non-small Cell Lung Carcinoma

SUBMITTER: Yi-Ju Chen  

LAB HEAD: Yu-Ju Chen

PROVIDER: PXD010456 | Pride | 2022-02-25

REPOSITORIES: pride

Json Xml

Dataset's files

Source:
Action DRS
YJC_151128_200ugPC9mem_cH0_G.raw Raw
YJC_151128_200ugPC9mem_cH0_G.raw_20200108_Byonic.mgf Mgf
YJC_151128_200ugPC9mem_cH0_G.raw_20200108_Byonic.mzid.gz Mzid
YJC_151128_200ugPC9mem_cH55_G.raw Raw
YJC_151128_200ugPC9mem_cH55_G.raw_20200108_Byonic.mgf Mgf
Items per page:
1 - 5 of 60
altmetric image

Publications

ZIC-cHILIC-Based StageTip for Simultaneous Glycopeptide Enrichment and Fractionation toward Large-Scale N-Sialoglycoproteomics.

Chen Yi-Ju YJ   Yen Ta-Chi TC   Lin Yu-Hsien YH   Chen Yan-Lin YL   Khoo Kay-Hooi KH   Chen Yu-Ju YJ  

Analytical chemistry 20211115 48

Alterations of protein glycosylation are closely related with pathophysiological regulation. Due to the structural macro- and microheterogeneity, low stoichiometry, and low ionization efficiency of glycopeptides, high-performance tools to enrich glycopeptides, especially the negatively charged and labile sialoglycopeptides, are essential to enhance the identification of the underexplored glycoproteome. Here, we present the first implementation of zwitterionic hydrophilic interaction chromatograp  ...[more]

Similar Datasets

Proteomics Human Breast LC-MSMS - Quantitative site- and structure-specific N-glycoproteomics characterization of differential N-glycosylation in MCF-7/ADR cancer stem cells
2020-02-21 | PXD013836 | Pride
Proteomics TIHAMAC: Streamlined Tandem IMAC-HILIC Workflow for Simultaneous and High-Throughput Plant Phosphoproteomics and N-glycoproteomics Analysis
2024-04-03 | PXD047065 | Pride
Proteomics CSF glycoproteomics reveals brain-specific changes in glyco-sylation in congenital disorders of glycosylation
2023-01-18 | PXD038787 | Pride
Proteomics Boost-DiLeu: Enhanced Isobaric N,N-Dimethyl Leucine Tagging Strategy for Comprehensive Quantitative Glycoproteomic Analysis
2022-09-08 | PXD029269 | Pride
Proteomics SARS-CoV-2 spike protein glycosylation enriched by HILIC and Ti-IMAC
2022-01-11 | PXD026450 | Pride
Proteomics Hydrophilic interaction liquid chromatography contributes to integrated N-glycoproteomics analysis of human saliva for lung cancer
2023-02-08 | PXD030670 | Pride
Proteomics Comparison of Intact Glycopeptide Enrichment Methods Using Strong Anion Exchange and Hydrophilic Interaction Liquid Chromatography
2017-10-19 | PXD005145 | Pride
Proteomics On-tissue spatially-resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscape
2022-05-04 | PXD025537 | Pride
Proteomics Detecting Proteins Glycosylation by a Homogeneous Reac-tion System with Zwitterionic Gold Nanoclusters
2017-03-29 | PXD005635 | Pride
Proteomics Intact N-glycopeptide signatures of immunoglobulin G molecules in plasma from patients with prostate carcinoma and benign prostatic hyperplasia
2020-06-29 | PXD016175 | Pride