Proteomics

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Human Breast LC-MSMS - Quantitative site- and structure-specific N-glycoproteomics characterization of differential N-glycosylation in MCF-7/ADR cancer stem cells

ABSTRACT: Cancer stem cells (CSCs) are reported to be responsible for tumor initiation, progression, metastasis, and therapy resistance where P-glycoprotein (P-gp) as well as other glycoproteins are involved. Identification of these glycoprotein markers is critical for understanding the resistance mechanism and developing therapeutics. Here we report our comparative N-glycoproteomics study of MCF‐7/ADR vs. MCF-7 cells. With zic-HILIC enrichment, isotopic diethyl labeling, RPLC-MS/MS (HCD) analysis and GPSeeker DB search, differentially expressed N-glycosylation was quantitatively characterized at the intact N-glycopeptides levels.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Yue Wang  

LAB HEAD: Zhixin Tian

PROVIDER: PXD013836 | Pride | 2020-02-21

REPOSITORIES: Pride

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Dataset's files

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Action DRS
GPs_ADR_MCF7_CSC_SIDE_400ug_20190122_NCE203040_1.raw Raw
GPs_ADR_MCF7_CSC_SIDE_400ug_20190122_NCE203040_2.raw Raw
GPs_ADR_MCF7_CSC_SIDE_400ug_20190122_NCE203040_3.raw Raw
SupplementaryTable1.IdentificationlistofMCF7-ADRCSCsandMCF7-ADR.xlsx Xlsx
SupplementaryTable2.QuantitationlistofMCF7-ADRCSCsandMCF7-ADR.xlsx Xlsx
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Publications

Quantitative site- and structure-specific N-glycoproteomics characterization of differential N-glycosylation in MCF-7/ADR cancer stem cells.

Xu Feifei F   Wang Yue Y   Xiao Kaijie K   Hu Yechen Y   Tian Zhixin Z   Chen Yun Y  

Clinical proteomics 20200205

<h4>Background</h4>Cancer stem cells (CSCs) are reported to be responsible for tumor initiation, progression, metastasis, and therapy resistance where P-glycoprotein (P-gp) as well as other glycoproteins are involved. Identification of these glycoprotein markers is critical for understanding the resistance mechanism and developing therapeutics.<h4>Methods</h4>In this study, we report our comparative and quantitative site- and structure-specific N-glycoproteomics study of MCF-7/ADR cancer stem ce  ...[more]

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