Dataset Information

Mucin-binding proteins of E. coli membrane

ABSTRACT: Proteins from E. coli outer membrane were extracted with carbonate method, separated in 2D-PAGE and transferred onto nitrocellulose membrane. After incubation with flourescent-labelled mucin, mucin-binding protein spots were cut out, subjected to trypsinolysis and identified by protein analysis with HPLC-MS-MS.

INSTRUMENT(S): maXis

ORGANISM(S): Escherichia Coli

SUBMITTER: Daria Rakitina

LAB HEAD: Gleb Yurievich Fisunov

PROVIDER: PXD010472 | Pride | 2019-12-16

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	2D-01-F032122.mzid.gz	Mzid
	2D-01-F032122.pride.mztab.gz	Mztab
	2D-02-F032123.mzid.gz	Mzid
	2D-02-F032123.pride.mztab.gz	Mztab
	2D-03-F032124.mzid.gz	Mzid

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Publications

Mucin adsorbed by E. coli can affect neutrophil activation in vitro.

Mikhalchik Elena E Balabushevich Nadezhda N Vakhrusheva Tatiana T Sokolov Alexey A Baykova Julia J Rakitina Daria D Scherbakov Petr P Gusev Sergey S Gusev Alexander A Kharaeva Zaira Z Bukato Olga O Pobeguts Olga O

FEBS open bio 20191219 2

Bacteria colonizing human intestine adhere to the gut mucosa and avoid the innate immune system. We previously demonstrated that Escherichia coli isolates can adsorb mucin from a diluted solution in vitro. Here, we evaluated the effect of mucin adsorption by E. coli cells on neutrophil activation in vitro. Activation was evaluated based on the detection of reactive oxygen species production by a chemiluminescent reaction (ChL), observation of morphological alterations in neutrophils and detectio ...[more]

PMID: 31785127

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