Proteomics

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Plant-derived virus-like particle vaccines drive cross-presentation of influenza A hemagglutinin peptides by human monocyte-derived macrophages


ABSTRACT: A growing body of evidence supports the importance of T cell responses to protect against severe influenza, promote viral clearance and ensure long-term immunity. Plant-derived virus-like particle (VLP) vaccines bearing influenza hemagglutinin (HA) have been shown to elicit strong humoral and CD4+ T cell responses in both pre-clinical and clinical studies. To better understand the immunogenicity of theses vaccines, we tracked the intracellular fate of a model HA (A/California/07/2009 H1N1) in human monocyte-derived macrophages (MDMs) following delivery either as VLPs (H1-VLP) or in soluble form. High-resolution tandem mass spectrometry identified 131 HA-derived peptides associated with MHC I in the H1-VLP-treated MDMs. Together with immunostaining and microscopy results, these data suggest that HA delivery to antigen-presenting cells on plant-derived VLPs facilitates antigen uptake, endosomal processing and cross-presentation. These observations may help explain the broad and cross-reactive immune responses generated by these vaccines.

INSTRUMENT(S): maXis

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte, Blood, Macrophage

DISEASE(S): Influenza

SUBMITTER: Momar Ndao  

LAB HEAD: Brian J. Ward

PROVIDER: PXD010519 | Pride | 2019-05-28

REPOSITORIES: Pride

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Publications

Plant-derived virus-like particle vaccines drive cross-presentation of influenza A hemagglutinin peptides by human monocyte-derived macrophages.

Makarkov Alexander I AI   Golizeh Makan M   Ruiz-Lancheros Elizabeth E   Gopal Angelica A AA   Costas-Cancelas Ian N IN   Chierzi Sabrina S   Pillet Stephane S   Charland Nathalie N   Landry Nathalie N   Rouiller Isabelle I   Wiseman Paul W PW   Ndao Momar M   Ward Brian J BJ  

NPJ vaccines 20190515


A growing body of evidence supports the importance of T cell responses to protect against severe influenza, promote viral clearance, and ensure long-term immunity. Plant-derived virus-like particle (VLP) vaccines bearing influenza hemagglutinin (HA) have been shown to elicit strong humoral and CD4<sup>+</sup> T cell responses in both pre-clinical and clinical studies. To better understand the immunogenicity of these vaccines, we tracked the intracellular fate of a model HA (A/California/07/2009  ...[more]

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