Dataset Information

MHC I-restricted immunopeptidomics analysis of human monocyte-derived macrophages exposed to biological nanoparticles bearing influenza hemagglutinin

ABSTRACT: In this study, primary human monocyte-derived macrophages were exposed to plant-derived virus-like particles (VLPs) bearing influenza A hemagglutinin (HA) or soluble influenza HA, as control. Immunopurified MHC class I-associated peptides were analysed by nano-flow high pressure liquid chromatography coupled to high-resolution dopant-assisted electrospray ionisation mass spectrometry. A total of 109 host-derived MHC I peptides were identified in the VLP-treated samples, two of which were also detected in controls. The peptides unique to VLP treatment were, on average, ~13 amino acid residues long, more basic and hydrophilic, and were mainly processed via proteolysis by matrix metalloproteinases and cathepsins. The proteins associated with these peptides were primarily involved in cellular, metabolic and regulatory processes and activated several pathways including inflammation stimulation and attenuation, response to stimuli, innate and adaptive immunity, clathrin-mediated endocytosis, protein synthesis and endo-lysosomal degradation. This study is the first report to describe the response of a primary human antigen-presenting cell to nanoparticulate vs. soluble antigen exposure from an immunopeptidomics point of view.

INSTRUMENT(S): maXis

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte, Blood, Macrophage

DISEASE(S): Influenza

SUBMITTER: Momar Ndao

LAB HEAD: Momar Ndao

PROVIDER: PXD010741 | Pride | 2022-02-24

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 26

Similar Datasets

Project description:Human papilloma (HPV) virus-like particle (VLP) vaccines were recently licensed. Though neutralizing antibody titers are thought to be the main effectors of protection against infection, early predictors of long-term efficacy are not yet defined and a comprehensive understanding of innate and adaptive immune responses to vaccination is still lacking. Here, microarrays were used to compare the gene expression signature in HPV-16 L1 VLP-stimulated PBMC from 17 vaccine and 4 placebo recipients before vaccination, and 1 month after receiving the second immunization. Vaccination with HPV-16 L1 VLP was associated with modulation of genes involved in the inflammatory/defense response, cytokine, interferon and cell cycle pathways in VLP-stimulated PBMC. In addition, there was up-regulation of probesets associated with cytotoxic (GZMB, TNFSF10) and regulatory (INDO, CTLA4) activities. The strongest correlations with neutralizing antibody titers were found for cyclin d2 (CCND2) and galectin (LGALS2). Twenty-two differentially expressed probesets were selected for confirmation by RT-PCR in an independent sample set. Agreement with the microarray data was seen for over two-thirds of these probesets. Up-regulation of immune/defense response genes by VLP, in particular interferon-induced genes was observed in PBMC collected prior to vaccination, with many of these genes being further induced following vaccination. In conclusion, we used gene expression profiling to identify important innate and adaptive response related- genes induced by vaccination with HPV VLP. Further studies are needed to identify gene expression signatures of immunogenicity and long-term protection with potential utility in prediction of long-term HPV vaccination outcomes in clinical trials. Experiment Overall Design: Microarrays (Affymetrix Human Focus) were used to compare the gene expression signature in PBMCs stimulated for 3 days with media alone, Sf9/baculovirus insect cell lysate, and HPV-16 L1 VLP expressed from baculovirus-infected Sf9 insect cells from 17 vaccine and 4 placebo recipients before vaccination, and 1 month after receiving the second immunization (2 months after the initial immunization. Post-vaccination baculovirus sample for Pt078 and pre-vaccination baculovirus sample for Pt082 failed QC.

			Action	DRS
	Sample0.zip	Other
	Sample1.zip	Other
	Sample2.zip	Other
	Sample3.zip	Other
	Sample4.zip	Other

Dataset Information

MHC I-restricted immunopeptidomics analysis of human monocyte-derived macrophages exposed to biological nanoparticles bearing influenza hemagglutinin

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets