Dataset Information

Proteomics Analysis of Escherichia coli Persisters

ABSTRACT: Persisters, a dormant and multi-drug tolerant subpopulation that are able to resuscitate after antibiotic treatment, have recently received considerable attentions as the major risk of the relapse of various infectious diseases in clinics. However, due to their low abundance and inherent mutability, it is extremely difficult to study them by proteomics. Here, we developed a magnetic beads-based separation approach to enrich Escherichia coli persisters and then subject them to Filter-Aided Sample Perparation (FASP) followed by LC-MS/MS analysis. We applied spectral counting-based quantitative proteomics to study the proteomic changes of E. coli persisters under high concentration of ampicillin treatment.

INSTRUMENT(S): LTQ

ORGANISM(S): Escherichia Coli

SUBMITTER: Jordy Evan Sulaiman

LAB HEAD: Henry Lam

PROVIDER: PXD010590 | Pride | 2018-10-16

REPOSITORIES: Pride

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Publications

Specific Enrichment and Proteomics Analysis of Escherichia coli Persisters from Rifampin Pretreatment.

Sulaiman Jordy Evan JE Hao Chunlin C Lam Henry H

Journal of proteome research 20181018 11

Bacterial persisters, a dormant and multidrug tolerant subpopulation that are able to resuscitate after antibiotic treatment, have recently received considerable attention as a major cause of relapse of various infectious diseases in the clinic. However, because of their low abundance and inherent transience, it is extremely difficult to study them by proteomics. Here we developed a magnetic-beads-based separation approach to enrich Escherichia coli persisters and then subjected them to shotgun ...[more]

PMID: 30336045

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	ARi1-2.mzML	Mzml
	ARi1-2.pep.xml	Pepxml