Dataset Information

Anoxygenic Photosynthesis and Dark Carbon Metabolism in the Purple Sulfur Bacterium “Thiodictyon syntrophicum“ nov. strain Cad16T

ABSTRACT: Strain Cad16T was previously shown to fix a major fraction of the bulk CO2 under both light and dark conditions and was suggested to be involved in Fe(II) driven aerobic metabolism in the lake Cadagno chemocline. However evidence on genomic and proteomic level was only fragmentary . In order to understand the biological functions expressed under phototrophic and chemotrophic growth conditions of str. Cad16T an in situ experiment conceived. It included cultures of str. Cad16T incubated in dialysis bags for three months while physiochemical conditions were monitored longitudinally. Upon sampling, physiological differences of str. Cad16T were compared utilizing quantitative proteomics in combination with CO2-uptake measurements.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Candidatus Thiodictyon Syntrophicum Str. Cad16

TISSUE(S): Cell Suspension Culture

SUBMITTER: Samuel Luedin

LAB HEAD: Mauro Tonolla

PROVIDER: PXD010641 | Pride | 2019-03-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20171006_03_62233_cad16t_dia_5_e1 from MAXQUANT_ 20171006_11_62239_cad16t_dia_11_e1 and 10 more.pride.mztab.gz	Mztab
	20171006_03_62233_cad16t_dia_5_e1.raw	Raw
	20171006_03_62233_cad16t_dia_5_e1__from__MAXQUANT__20171006_11_62239_cad16t_dia_11_e1_and_10_more.MGF	Mgf
	20171006_03_62233_cad16t_dia_5_e1__from__MAXQUANT__20171006_11_62239_cad16t_dia_11_e1_and_10_more.pride.mgf.gz	Mgf
	20171006_03_62233_cad16t_dia_5_e1fromMAXQUANT_20171006_11_62239_cad16t_dia_11_e1and10more.mzid.gz	Mzid

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Publications

Mixotrophic Growth Under Micro-Oxic Conditions in the Purple Sulfur Bacterium "Thiodictyon syntrophicum".

Luedin Samuel M SM Storelli Nicola N Danza Francesco F Roman Samuele S Wittwer Matthias M Pothier Joël F JF Tonolla Mauro M

Frontiers in microbiology 20190305

The microbial ecosystem of the meromictic Lake Cadagno (Ticino, Swiss Alps) has been studied intensively in order to understand structure and functioning of the anoxygenic phototrophic sulfur bacteria community living in the chemocline. It has been found that the purple sulfur bacterium "Thiodictyon syntrophicum" strain Cad16T, belonging to the Chromatiaceae, fixes around 26% of all bulk inorganic carbon in the chemocline, both during day and night. With this study, we elucidat ...[more]

PMID: 30891015

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Project description:Development of systems allowing the maintenance of native properties of mesenchymal stromal cells (MSC) is a critical challenge for studying physiological functions of skeletal progenitors, as well as towards cellular therapy and regenerative medicine applications. Conventional stem cell culture in monolayer on plastic dishes (2D) is associated with progressive loss of functionality, likely due to the absence of a biomimetic microenvironment and the selection of adherent populations. Here we demonstrate that 2D MSC expansion can be entirely bypassed by culturing freshly isolated bone marrow cells within the pores of 3D scaffolds in a perfusion-based bioreactor system, followed by enzymatic digestion for cell retrieval. The 3D-perfusion system supported MSC growth while maintaining cells of the hematopoietic lineage, and thus generated a cellular environment mimicking some features of the bone marrow stroma. As compared to 2D-expansion, sorted CD45- cells derived from 3D-perfusion culture after the same time (3 weeks) or a similar extent of proliferation (7-8 doublings) maintained a 4.3-fold higher clonogenicity and exhibited a superior differentiation capacity towards all typical mesenchymal lineages, with similar immunomodulatory function in vitro. Transcriptomic analysis performed on MSC from 5 donors validated the robustness of the process and indicated a reduced inter-donor variability as well as a significant upregulation of multipotency-related gene clusters following 3D-perfusion as compared to 2D expansion. The described system offers a model to study how factors of a 3D engineered niche may regulate MSC function and, by streamlining conventional labor-intensive processes, is prone to automation and scalability within closed bioreactor systems. Nucleated cells were isolated from 5 fresh human bone marrow aspirates by means of red blood cells lyses buffer and then were seeded into a 3D perfusion bioreactor system using a pure hydroxyapatite 3D scaffold and in conventional Petri dishes (2D). After culture for 19 days, cells from both systems were enzymatically retrieved and sorted using anti-CD45-coated magnetic beads. Total RNA was extracted from CD45- cells, QCed and hybridized to Affymetrix microarrays.

Dataset Information

Anoxygenic Photosynthesis and Dark Carbon Metabolism in the Purple Sulfur Bacterium “Thiodictyon syntrophicum“ nov. strain Cad16T

Dataset's files

Publications

Mixotrophic Growth Under Micro-Oxic Conditions in the Purple Sulfur Bacterium "<i>Thiodictyon syntrophicum</i>".

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