Proteomics

Dataset Information

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Bacteriocins in Lactobacillus acidophlius membrane vesicles


ABSTRACT: Recent reports have shown that Gram-positive bacteria actively secrete spherical nanometer-sized proteolipid membrane vesicles (MVs) into their surroundings. Though MVs have been implicated in a broad range of biological functions, few studies have been conducted to examine their potential as delivery vehicles of antimicrobials. Here, we investigate the natural ability of Lactobacillus acidophilus MVs to carry and deliver bacteriocin peptides to the opportunistic pathogen, Lactobacillus delbrueckii. We demonstrate that upon treatment with lactacin B-inducing peptide the proteome of the secreted MVs is enriched in putative bacteriocins encoded by the lab operon. Further, we show that purified MVs inhibit growth and form membrane pores in L. delbrueckii, which is confirmed using a number of microscopy techniques and spectrophotometry. These results show that L. acidophilus MVs serve as conduits for antimicrobials to competing cells in the environment, suggesting a potential role for MVs in complex communities such as the gut microbiome. With the potential for controlling their payload through microbial engineering, MVs produced by L. acidophilus may be an interesting platform for effecting change in complex microbial communities or aiding in the development of new biomedical therapeutics.

INSTRUMENT(S): LTQ Orbitrap, TripleTOF 5600

ORGANISM(S): Lactobacillus Acidophilus Ncfm

SUBMITTER: Scott Dean  

LAB HEAD: Scott Walper

PROVIDER: PXD012967 | Pride | 2020-03-20

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
MA_UASML_Ng0_1.mgf Mgf
MA_UASML_Ng0_1.mzid.gz Mzid
MA_UASML_Ng0_1.pride.mgf.gz Mgf
MA_UASML_Ng0_1.pride.mztab.gz Mztab
MA_UASML_Ng0_1.raw Raw
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Publications

<i>Lactobacillus acidophilus</i> Membrane Vesicles as a Vehicle of Bacteriocin Delivery.

Dean Scott N SN   Rimmer Mary Ashley MA   Turner Kendrick B KB   Phillips Daniel A DA   Caruana Julie C JC   Hervey William Judson WJ   Leary Dagmar H DH   Walper Scott A SA  

Frontiers in microbiology 20200430


Recent reports have shown that Gram-positive bacteria actively secrete spherical nanometer-sized proteoliposome membrane vesicles (MVs) into their surroundings. Though MVs are implicated in a broad range of biological functions, few studies have been conducted to examine their potential as delivery vehicles of antimicrobials. Here, we investigate the natural ability of <i>Lactobacillus acidophilus</i> MVs to carry and deliver bacteriocin peptides to the opportunistic pathogen, <i>Lactobacillus d  ...[more]

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