Proteomics

Dataset Information

0

Chlamydomonas reinhardtii flagella cc125 ift139-2 dhc1b-3


ABSTRACT: As part of a cryo EM study of IFT trains in Chlamydomonas flagella we used two mutants with defects in IFT139 or DHC1b. To determine what proteins were missing in the flagella of the mutants we did a proteomic analysis of the membrane matrix fraction from flagella isolated from the mutants.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Chlamydomonas Reinhardtii

TISSUE(S): Plant Cell

DISEASE(S): Disease Free

SUBMITTER: Dennis Diener  

LAB HEAD: Gaia Pigino

PROVIDER: PXD010699 | Pride | 2018-10-19

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
3-samples-20171017.sf3 Other
dhc.mzid.gz Mzid
dhc.mzid_dhc.MGF Mzid
dhc.mzid_dhc.pride.mgf.gz Mzid
dhc.pride.mztab.gz Mztab
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Publications

The cryo-EM structure of intraflagellar transport trains reveals how dynein is inactivated to ensure unidirectional anterograde movement in cilia.

Jordan Mareike A MA   Diener Dennis R DR   Stepanek Ludek L   Pigino Gaia G  

Nature cell biology 20181015 11


Movement of cargos along microtubules plays key roles in diverse cellular processes, from signalling to mitosis. In cilia, rapid movement of ciliary components along the microtubules to and from the assembly site is essential for the assembly and disassembly of the structure itself<sup>1</sup>. This bidirectional transport, known as intraflagellar transport (IFT)<sup>2</sup>, is driven by the anterograde motor kinesin-2<sup>3</sup> and the retrograde motor dynein-1b (dynein-2 in mammals)<sup>4,5  ...[more]

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