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The GFZF protein interactome in Drosophila melanogaster 0-12h old embryos

ABSTRACT: The gfzf gene is essential for viability and it has been identified in several genetic screenings pointed out its importance in different cellular processes. However, its molecular function has not been extensively studied so far. Here we analyse the GFZF protein interactome in Drosophila melanogaster embryos. The GFZF protein was subjected to immunoprecipitation with an antibody against the endogenous protein and the interactome was analysed by mass spectrometry. The samples are named as follows: antibody used _ batch _ MS ID _ date of MS run

INSTRUMENT(S): Q Exactive

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Embryo

SUBMITTER: Ignasi Forne

LAB HEAD: Axel Imhof

PROVIDER: PXD010712 | Pride | 2019-11-12

REPOSITORIES: Pride

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			Action	DRS
	GFZF_1_ALMS337_20170825_.raw	Raw
	GFZF_2_ALMS347_20180202.raw	Raw
	GFZF_3_ALMS353_20180202.raw	Raw
	GFZF_4_ALMS379_20180628.raw	Raw
	IgG_1_ALMS340335_20170825.raw	Raw

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Publications

Altered Localization of Hybrid Incompatibility Proteins in Drosophila.

Cooper Jacob Carter JC Lukacs Andrea A Reich Shelley S Schauer Tamas T Imhof Axel A Phadnis Nitin N

Molecular biology and evolution 20190801 8

Understanding the molecular basis of hybrid incompatibilities is a fundamental pursuit in evolutionary genetics. In crosses between Drosophila melanogaster females and Drosophila simulans males, an interaction between at least three genes is necessary for hybrid male lethality: Hmr mel, Lhr sim, and gfzf sim. Although HMR and LHR physically bind each other and function together in a single complex, the connection between gfzf and either of these proteins remains mysterious. Here, we show that GF ...[more]

PMID: 31038678

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Project description:In this study we described the protein-protein interaction network of the Drosophila Speciation Core Complex by analysing the interactome of its subunit: HMR, LHR, NLP, BOH1 (CG33213), BOH2 (CG4788) and HP1a. For this purpose we performed Affinity Purification coupled with Mass Spectrometry (AP-MS) in D. melanogaster SL2 cells using as bait the two hybrid incompatibility proteins HMR (n = 8) and LHR (n = 4), as well as NLP (n = 3), BOH1(CG33213, n = 4), BOH2 (CG4788, n = 5) and HP1a (n = 4). Each bait was targeted with at least one antibody (rat anti-LHR 12F4, mouse anti-HP1a 2C09, rabbit anti-Nlp, anti-FLAG-M2 for FLAG-CG33213 and FLAG-CG4788), while HMR was targeted with three different antibodies (rat anti-HMR 2C10 and 12F1, anti-FLAG-M2 for FLAG-HMR). Individual replicates and antibodies used are listed in samples_table.

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