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Characterization of extracellular enzymes involved in natural decomposition of conifer wood

ABSTRACT: The conifer needle endophyte, Phialocephala scopiformis, was cultivated in media containing ground Pinus contorta wood as sole carbon source. After five and seven days growth, concentrated extracellular fluids were subjected to LC-MS/MS analyses.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Phialocephala Scopiformis

SUBMITTER: Greg Sabat

LAB HEAD: Dr. Daniel Cullen

PROVIDER: PXD010720 | Pride | 2018-11-28

REPOSITORIES: pride

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			Action	DRS
	Day-5_Cullen.raw	Raw
	Day-5_Cullen_F017728.mzid	Mzid
	Day-5_Cullen_F017728.mzid_Day-5_Cullen_F017728.MGF	Mzid
	Day-7_Cullen.raw	Raw
	Day-7_Cullen_F017729.mzid	Mzid

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Publications

The Foliar Endophyte <i>Phialocephala scopiformis</i> DAOMC 229536 Proteome When Grown on Wood Used as the Sole Carbon Source.

Bhatnagar Jennifer M JM Sabat Grzegorz G Cullen Daniel D

Microbiology resource announcements 20190207 6

The conifer needle endophyte Phialocephala scopiformis DAOMC 229536 was cultivated in medium containing ground Pinus contorta wood as the sole carbon source. Mass spectrometry analyses identified 590 proteins. The expression of extracellular hydrolases and oxidoreductases indicates a capacity to degrade wood. The results clearly demonstrate the latent saprophytic potential of P. scopiformis. ...[more]

PMID: 30746512

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