Dataset Information

Quantitative proteomics of immunocaptured mitochondria from Purkinje Cells

ABSTRACT: Mitochondria vary in morphology and function in different tissues, however little is known about their molecular diversity among cell types. To investigate mitochondrial diversity in vivo, we developed an efficient protocol to isolate cell type-specific mitochondria based on a new MitoTag mouse. We profiled the mitochondrial proteome of three major neural cell types in cerebellum and identified a substantial number of differential mitochondrial markers for these cell types in mice and humans. Based on predictions from these proteomes, we demonstrate that astrocytic mitochondria metabolize long-chain fatty acids more efficiently than neurons. Moreover, we identified Rmdn3 as a major determinant of ER-mitochondria proximity in Purkinje cells. Our novel approach enables exploring mitochondrial diversity on the functional and molecular level in many in vivo contexts.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Purkinje Cell

SUBMITTER: Stephan Mueller

LAB HEAD: Thomas Misgeld

PROVIDER: PXD010772 | Pride | 2019-09-03

REPOSITORIES: Pride

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Publications

Cell-type-specific profiling of brain mitochondria reveals functional and molecular diversity.

Fecher Caroline C Trovò Laura L Müller Stephan A SA Snaidero Nicolas N Wettmarshausen Jennifer J Heink Sylvia S Ortiz Oskar O Wagner Ingrid I Kühn Ralf R Hartmann Jana J Karl Rosa Maria RM Konnerth Arthur A Korn Thomas T Wurst Wolfgang W Merkler Doron D Lichtenthaler Stefan F SF Perocchi Fabiana F Misgeld Thomas T

Nature neuroscience 20190909 10

Mitochondria vary in morphology and function in different tissues; however, little is known about their molecular diversity among cell types. Here we engineered MitoTag mice, which express a Cre recombinase-dependent green fluorescent protein targeted to the outer mitochondrial membrane, and developed an isolation approach to profile tagged mitochondria from defined cell types. We determined the mitochondrial proteome of the three major cerebellar cell types (Purkinje cells, granule cells and as ...[more]

PMID: 31501572

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