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Peptide mapping of purified huntingtin protein samples from different eukaryotic expression systems

ABSTRACT: Huntingtin expression, purification and biophysical characterisation: purified huntingtin protein samples of different polyQ lengths derived from insect cell and mammalian cell production were digested and subject to peptide mass spectrometry.

INSTRUMENT(S): 6545 Q-TOF LC/MS

ORGANISM(S): Homo Sapiens (human) Spodoptera Frugiperda

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Huntington Disease

SUBMITTER: Suzanne Ackloo

LAB HEAD: Cheryl H Arrowsmith

PROVIDER: PXD010865 | Pride | 2019-03-12

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Cont-trypsin_Htt_HEK293_2.mgf	Mgf
	Cont-trypsin_Htt_HEK293_2.mzxml	Mzxml
	Cont-trypsin_Htt_HEK293_2.pep.xml	Pepxml
	Cont-trypsin_Htt_HEK293_2.pep.xml.zip	Pepxml
	Digesttest-22mingradientQ19HEK3195.mgf	Mgf

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Publications

Design and characterization of mutant and wildtype huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems.

Harding Rachel J RJ Loppnau Peter P Ackloo Suzanne S Lemak Alexander A Hutchinson Ashley A Hunt Brittany B Holehouse Alex S AS Ho Jolene C JC Fan Lixin L Toledo-Sherman Leticia L Seitova Alma A Arrowsmith Cheryl H CH

The Journal of biological chemistry 20190306 17

The gene mutated in individuals with Huntington's disease (HD) encodes the 348-kDa huntingtin (HTT) protein. Pathogenic HD CAG-expansion mutations create a polyglutamine (polyQ) tract at the N terminus of HTT that expands above a critical threshold of ∼35 glutamine residues. The effect of these HD mutations on HTT is not well understood, in part because it is difficult to carry out biochemical, biophysical, and structural studies of this large protein. To facilitate such studies, here we have ge ...[more]

PMID: 30842263

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Analysis of the Zur Regulon of Moraxella catarrhalis

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2014-03-16 | E-GEOD-28027 | biostudies-arrayexpress

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G3BP1-dependent suppression of polyglutamine aggregation in Huntington’s models and its failure upon stress granule formation

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