Proteomics

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Micro-proteomics experiment for single meiotic-phase barley anthers

ABSTRACT: We describe here a new micro-proteomics workflow for the analysis of individual barley anthers. The workflow detects more than 4000 proteins from small amounts of material representing single or paired anther samples, covering a dynamic range of protein expression levels across five orders of magnitude. We consistently identified ~2800 proteins in a single 0.6mm anther and ~4000 proteins in a paired-anther sample. This demonstrates an important technological advance in plant proteomics, considering the limited amount of starting material. We employed our micro-proteomics workflow to investigate the proteome of the developing barley anther containing male gametes in the early stages of meiosis. We successfully identified several low abundant meiosis-related proteins, proving that our approach is highly sensitive and consequently powerful. To date, no work has been published previously on precisely staged individual plant anthers.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Hordeum Vulgare (barley)

TISSUE(S): Anther

SUBMITTER: Runxuan Zhang  

LAB HEAD: Robbie Waugh

PROVIDER: PXD010887 | Pride | 2019-05-04

REPOSITORIES: Pride

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Andromeda.zip Other
Andromeda_1Anther.zip Other
Andromeda_2Anthers.zip Other
Andromeda_5Anthers.zip Other
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Publications

Application of a Sensitive and Reproducible Label-Free Proteomic Approach to Explore the Proteome of Individual Meiotic-Phase Barley Anthers.

Lewandowska Dominika D   Zhang Runxuan R   Colas Isabelle I   Uzrek Nicola N   Waugh Robbie R  

Frontiers in plant science 20190402

Meiosis is a highly dynamic and precisely regulated process of cell division, leading to the production of haploid gametes from one diploid parental cell. In the crop plant barley (<i>Hordeum vulgare</i>), male meiosis occurs in anthers, in specialized cells called meiocytes. Barley meiotic tissue is scarce and not easily accessible, making meiosis study a challenging task. We describe here a new micro-proteomics workflow that allows sensitive and reproducible genome-wide label-free proteomic an  ...[more]

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