Proteomics

Dataset Information

0

The proteome of developing barley anthers during meiotic prophase I

ABSTRACT: In the current project, we aimed to discover how the barley anther proteome changes, both quantitatively and qualitatively, during meiotic prophase I when the important process of homologous recombination is taking place. Taking advantage of a recently published barley anther transcriptomic dataset (BAnTr) and a newly developed sensitive mass spectrometry-based approach to analyse barley anther proteome, we have conducted high-resolution mass spectrometry analysis of barley anthers, collected at 6 time points and representing their development from pre-meiosis to metaphase. Each time point was carefully staged using immunocytology. We identified >6400 proteins, including 82 known and putative meiotic proteins, and were able to quantify dynamic variation in the proteome during different stages of barley anther development.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Hordeum Vulgare (barley)

TISSUE(S): Anther, Plant Cell

SUBMITTER: Runxuan Zhang  

LAB HEAD: Robbie Waugh

PROVIDER: PXD026200 | Pride | 2022-04-04

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
DL-103A.raw Raw
DL-103B.raw Raw
DL-103C.raw Raw
DL-105A.raw Raw
DL-105B.raw Raw
Items per page:
1 - 5 of 91
altmetric image

Publications

The proteome of developing barley anthers during meiotic prophase I.

Lewandowska Dominika D   Orr Jamie J   Schreiber Miriam M   Colas Isabelle I   Ramsay Luke L   Zhang Runxuan R   Waugh Robbie R  

Journal of experimental botany 20220301 5

Flowering plants reproduce sexually by combining a haploid male and female gametophyte during fertilization. Male gametophytes are localized in the anthers, each containing reproductive (meiocyte) and non-reproductive tissue necessary for anther development and maturation. Meiosis, where chromosomes pair and exchange their genetic material during a process called recombination, is one of the most important and sensitive stages in breeding, ensuring genetic diversity. Most anther development stud  ...[more]

Similar Datasets

Proteomics micro-proteomics experiment for single meiotic-phase barley anthers
2019-05-04 | PXD010887 | Pride
Transcriptomics Identification of anther expressing genes in cotton (Gossypium hirsutum)
2013-10-31 | E-GEOD-51779 | biostudies-arrayexpress
Transcriptomics Uncovering small RNA-mediated responses to cold stress in a wheat thermosensitive genic male sterile line by deep sequencing
2012-03-29 | E-GEOD-36867 | biostudies-arrayexpress
Transcriptomics Uncovering small RNA-mediated responses to cold stress in a wheat thermosensitive genic male sterile line by deep sequencing (Degradome-Seq)
2012-04-11 | E-GEOD-37134 | biostudies-arrayexpress
Proteomics Unravelling the proteomic profile of rice meiocytes during early meiosis
2014-08-13 | PXD001058 | Pride
Proteomics Proteomic and Phosphoproteomic Analyses Reveal Extensive Phosphorylation of Regulatory Proteins in Developing Rice Anthers
2021-01-13 | PXD002756 | Pride
Transcriptomics RNA-seq data from spikelet tissues of cultivated two-row European spring barley genotypes.
2023-08-22 | E-MTAB-13231 | biostudies-arrayexpress
Proteomics Protein acetylation landscape of developing rice anthers
2019-03-06 | PXD007986 | Pride
Proteomics Proteomic Profiling of Barley Spent Grains
2018-03-23 | PXD008090 | Pride
Transcriptomics Identification of candidate non-host resistance genes for powdery mildew in barley
2014-10-24 | E-MTAB-2916 | biostudies-arrayexpress