Proteomics

Dataset Information

0

Identification of CTPS1 Filament Interacting Proteins

ABSTRACT: CTP synthase (CTPS) catalyzes the formation of CTP in de novo pyrimidine biosynthesis pathway. Compartmentalization of CTPS into filamentous structure is evolutionarily conserved from E.coli, yeast, Drosophila, mice to humans. Recently, we have demonstrated that histidine-mediated protein methylation promotes the formation of CTPS filament under glutamine and/or serum deprivation, which reduces its enzymatic activity and protects it from degradation. However, it is still unclear how the filament assembly takes place. In the current study, we used APEX2-mediated in vivo proximity labeling to identify proteins involved in CTPS filament formation.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Yu-Tsun Lin  

LAB HEAD: Jau-Song Yu

PROVIDER: PXD010921 | Pride | 2020-03-24

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
20161104-CTP-APEX2-s01.raw Raw
20161104-CTP-APEX2-s02.raw Raw
20161104-CTP-APEX2-s03.raw Raw
20161104-CTP-APEX2-s04.raw Raw
20161104-CTP-APEX2-s05.raw Raw
Items per page:
1 - 5 of 49
altmetric image

Publications

Under metabolic stress, cellular components can assemble into distinct membraneless organelles for adaptation. One such example is cytidine 5'-triphosphate synthase (CTPS, for which there are CTPS1 and CTPS2 forms in mammals), which forms filamentous structures under glutamine deprivation. We have previously demonstrated that histidine (His)-mediated methylation regulates the formation of CTPS filaments to suppress enzymatic activity and preserve the CTPS protein under glutamine deprivation, whi  ...[more]

Similar Datasets

Proteomics Identification of CTPS1 Filament Interacting Proteins_2019 dataset
2020-03-24 | PXD015507 | Pride
Transcriptomics DamX controls reversible cell morphology-switching in uropathogenic Escherichia coli.
2017-05-28 | E-MTAB-3611 | biostudies-arrayexpress
Genomics The INO80 complex removes H2A.Z to promote presynaptic filament formation during homologous recombination
2017-05-14 | GSE95801 | GEO
Transcriptomics Histidine-dependent protein methylation is required for compartmentalization of CTP synthase
2018-08-29 | GSE105251 | GEO
Transcriptomics Transcriptomes of the developing larval ovaries of Drosophila melanogaster during terminal filament formation
2021-10-18 | GSE172015 | GEO
Transcriptomics Microevolution of Candida albicans in macrophages restores filamentation in a nonfilamentous mutant
2015-03-31 | E-GEOD-56174 | biostudies-arrayexpress
Genomics Transcriptomes of the developing larval ovaries of Drosophila melanogaster during terminal filament formation
| PRJNA721702 | ENA
Proteomics Barbatia virescens byssal filament proteins
2024-05-17 | MSV000094783 | MassIVE
Transcriptomics Cell-specific gene expression in Anabaena variabilis grown phototrophically, mixotrophically, and heterotrophically
2013-11-01 | E-GEOD-46076 | biostudies-arrayexpress
Transcriptomics Candida albicans PPG1, a serine/threonine phosphatase, plays a vital role in central carbon metabolisms under filament-inducing conditions: A multi-omics approach
2024-04-10 | GSE263609 | GEO