Proteomics

Dataset Information

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Identification of CTPS1 Filament Interacting Proteins_2019 dataset

ABSTRACT: CTP synthase (CTPS) catalyzes the formation of CTP in de novo pyrimidine biosynthesis pathway. Compartmentalization of CTPS into filamentous structure is evolutionarily conserved from E.coli, yeast, Drosophila, mice to humans. Previously, we demonstrated that histidine (His) mediated methylation promotes the formation of CTPS filament to suppress the enzymatic activity and preserve CTPS protein under Gln deprivation, which promotes cancer cell growth after stress alleviation. However, it remains unclear where and how these enigmatic structures are assembled. Here we used APEX2-mediated in vivo proximity labeling to identify proteins involve in the formation of human CTPS filaments.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Yu-Tsun Lin  

LAB HEAD: Jau-Song Yu

PROVIDER: PXD015507 | Pride | 2020-03-24

REPOSITORIES: Pride

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Dataset's files

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20190809_APEX_TMT6_5seg.msf Msf
20190809_APEX_TMT6_5seg_s01.raw Raw
20190809_APEX_TMT6_5seg_s02.raw Raw
20190809_APEX_TMT6_5seg_s03.raw Raw
20190809_APEX_TMT6_5seg_s04.raw Raw
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Publications

Under metabolic stress, cellular components can assemble into distinct membraneless organelles for adaptation. One such example is cytidine 5'-triphosphate synthase (CTPS, for which there are CTPS1 and CTPS2 forms in mammals), which forms filamentous structures under glutamine deprivation. We have previously demonstrated that histidine (His)-mediated methylation regulates the formation of CTPS filaments to suppress enzymatic activity and preserve the CTPS protein under glutamine deprivation, whi  ...[more]

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