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Comparative proteomics using iodoTMT to identify changes in protein expression and cysteine oxidation in Leishmania mexicana

ABSTRACT: Two cell-lines of Leishmania mexicana were investigated using a TMT-based approach; wild-type and an attenuated H-line. Half of each sample was fully reduced and all cysteine residues were labelled with the cysteine-reactive iodoTMT reagent, while the other half was reacted with N-ethylmaleimide to block all free cysteines before the remaining reversibly modified cysteines were reduced and labelled with iodoTMT. This allowed direct comparisons to be made between the level of modified cysteines and the total cysteine count.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Leishmania Mexicana Mhom/gt/2001/u1103

TISSUE(S): Cell Culture

SUBMITTER: Aruna Prakash

LAB HEAD: Richard Burchmore

PROVIDER: PXD010938 | Pride | 2018-11-23

REPOSITORIES: Pride

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			Action	DRS
	Lmex_H_IP.mgf	Mgf
	Lmex_H_IP.pep.pep.xml	Pepxml
	Lmex_H_IP.prot.prot.xml	Xml
	Lmex_H_IP.raw	Raw
	Lmex_H_wash.mgf	Mgf

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