Dataset Information

Anticancer effect of deuterium depleted water - comprehensive proteomics analysis suggests a redox disbalance mechanism

ABSTRACT: The proteomics data is derived from 4 parts of samples prepared and analysed by nanoLC-MS/MS. In order to make appropriate statistical analysed could be performed, all treatments were performed in biological replicates. The number of replicates was 4 for protein abundances analysis (part 1), 2 for time course analysis (part 2), 3 for redox proteomics analysis (part 3) and 2 for TPP analysis (part 4). Each set of TMT10 labelling samples were combined and fractionationed into 10 fractions. In total, 113 TMT labelling samples including 11 sets of TMT10 and 3 sets of iodoTMT6 were performed with a 120 min gradient. In each part of the experiment, separate controls treated with the vehicle were included. Samples were analysed in random order to reduce the “order of injection” effect. In part 1, A549 cells were treated with CAMP, MTX, PCTL and 80 ppm DDW (20 samples). In part 2, the cells were treated 80 ppm DDW, MTX and PCTL for 4-48h (5 time points, 40 samples). In addition, 2 control samples treated with the vehicle for 48h were included in each set of TMT10 for normalization. In part 3, the cells were treated with 80 ppm DDW and auranofin (3 samples). Finally, in part 4, the cells were treated with 80 ppm DDW and afterwords the cells were treated with 10 temperatures (40 samples). Quality check was performed by calculating the variation (CV) between the replicates as well as by building PCA models to verify the small overall spread between the replicates.

INSTRUMENT(S): LTQ Orbitrap Elite, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Lung Cancer

SUBMITTER: Xuepei Zhang

LAB HEAD: Roman A Zubarev

PROVIDER: PXD011085 | Pride | 2019-09-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	DDW_expressionproteomics_RepA_frac1.raw	Raw
	DDW_expressionproteomics_RepA_frac10.raw	Raw
	DDW_expressionproteomics_RepA_frac2.raw	Raw
	DDW_expressionproteomics_RepA_frac3.raw	Raw
	DDW_expressionproteomics_RepA_frac4.raw	Raw

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Publications

Anticancer Effect of Deuterium Depleted Water - Redox Disbalance Leads to Oxidative Stress.

Zhang Xuepei X Gaetani Massimiliano M Chernobrovkin Alexey A Zubarev Roman A RA

Molecular & cellular proteomics : MCP 20190913 12

Despite the convincing empirical evidence that deuterium depleted water (DDW, 25-125 ppm deuterium) has anticancer effect, the molecular mechanism remains unclear. Here, redox proteomics investigation of the DDW action in A549 cells revealed an increased level of oxidative stress, whereas expression proteomics in combination with thermal profiling uncovered crucial role of mitochondrial proteins. In the proposed scenario, reversal of the normally positive deuterium gradient across the inner memb ...[more]

PMID: 31519768

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