Dataset Information

Epistasis studies reveal redundancy among calcium-dependent protein kinases in motility and invasion of malaria parasites

ABSTRACT: In malaria parasites, evolution of parasitism has been linked to functional optimisation. Despite this optimisation, most members of a calcium-dependent protein kinase (CDPK) family show genetic redundancy during erythrocytic proliferation. To identify relationships between phospho-signalling pathways, we here screen 294 genetic interactions among protein kinases in Plasmodium berghei. This reveals a synthetic negative interaction between a hypomorphic allele of the protein kinase G (PKG) and CDPK4 to control erythrocyte invasion which is conserved in P. falciparum. CDPK4 becomes critical when PKG-dependent calcium signals are attenuated to phosphorylate proteins important for the stability of the inner membrane complex, which serves as an anchor for the acto-myosin motor required for motility and invasion. Finally, we show that multiple kinases functionally complement CDPK4 during erythrocytic proliferation and transmission to the mosquito vector. This study reveals how CDPKs are wired within a stage-transcending signalling network to control motility and host cell invasion in malaria parasites.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Plasmodium Berghei Anka

DISEASE(S): Malaria

SUBMITTER: Mathieu Brochet

LAB HEAD: Mathieu Brochet

PROVIDER: PXD011096 | Pride | 2018-09-17

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	20160509_LU126_GAP40-HA_CDPK4-KO.MGF	Mgf
	20160509_LU126_GAP40-HA_CDPK4-KO.dta	Other
	20160509_LU126_GAP40-HA_CDPK4KO.raw	Raw
	20160812_QE97_CDPK1-HA.MGF	Mgf
	20160812_QE97_CDPK1-HA.dta	Other

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Publications

Epistasis studies reveal redundancy among calcium-dependent protein kinases in motility and invasion of malaria parasites.

Fang Hanwei H Gomes Ana Rita AR Klages Natacha N Pino Paco P Maco Bohumil B Walker Eloise M EM Zenonos Zenon A ZA Angrisano Fiona F Baum Jake J Doerig Christian C Baker David A DA Billker Oliver O Brochet Mathieu M

Nature communications 20181012 1

In malaria parasites, evolution of parasitism has been linked to functional optimisation. Despite this optimisation, most members of a calcium-dependent protein kinase (CDPK) family show genetic redundancy during erythrocytic proliferation. To identify relationships between phospho-signalling pathways, we here screen 294 genetic interactions among protein kinases in Plasmodium berghei. This reveals a synthetic negative interaction between a hypomorphic allele of the protein kinase G (PKG) and CD ...[more]

PMID: 30315162

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