PfCDPK1 mediated signaling in erythrocytic stages of Plasmodium falciparum

ABSTRACT: Calcium Dependent Protein Kinases (CDPKs) are key effectors of calcium signaling in malaria parasite. We have elucidated a novel role of PfCDPK1, which is indispensable for the erythrocytic development of Plasmodium falciparum, in invasion of host Red Blood Cells (RBCs). To gain insights into the mechanisms via which PfCDPK1 is involved in parasitic processes, a comparative phosphoproteomic analysis was employed to identify its parasitic targets. Proteins were isolated from CDPK1 knockdown and wild type parasites and processed for trypsin digestion and iTRAQ labeling. Quantitative proteomic analysis using high resolution mass spectrometry led to the identification of hypophosphorylated proteins, which can be the likely substrates for this kinase. In addition, in vitro kinase assays and antibody based validation of the hypophosphorylated sites was carried out. This study will provide a novel insight into the CDPK1 mediated invasion by Plasmodium falciparum.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Plasmodium Falciparum

TISSUE(S): Cell

DISEASE(S): Disease Free

SUBMITTER: Keshava Prasad T. S.  

LAB HEAD: Keshava Prasad

PROVIDER: PXD005207 | Pride | 2017-05-02

REPOSITORIES: Pride