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Universal Solid-Phase Protein Preparation (USP3) for bottom-up and top-down proteomics

ABSTRACT: Selecting a sample preparation strategy for mass spectrometry-based proteomics is critical to the success of quantitative workflows. Here we present a universal, solid-phase protein preparation (USP3) method which is rapid, robust and scalable, facilitating high-throughput protein sample preparation for bottom-up and top-down mass spectrometry (MS) analysis.

INSTRUMENT(S): Bruker Daltonics micrOTOF series

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Hela Cell

SUBMITTER: Laura Dagley

LAB HEAD: Andrew I Webb

PROVIDER: PXD011169 | Pride | 2019-06-05

REPOSITORIES: pride

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	P3493_A10_1_01_8697.d.zip	Other
	P3493_A11_1_01_8699.d.zip	Other
	P3493_A12_1_01_8701.d.zip	Other
	P3493_A1_1_01_8679.d.zip	Other
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Universal Solid-Phase Protein Preparation (USP<sup>3</sup>) for Bottom-up and Top-down Proteomics.

Dagley Laura F LF Infusini Giuseppe G Larsen Rune H RH Sandow Jarrod J JJ Webb Andrew I AI

Journal of proteome research 20190610 7

Selecting a sample preparation strategy for mass spectrometry-based proteomics is critical to the success of quantitative workflows. Here we present a universal, solid-phase protein preparation (USP<sup>3</sup>) method which is rapid, robust, and scalable, facilitating high-throughput protein sample preparation for bottom-up and top-down mass spectrometry (MS) analysis. This technique builds upon the single-pot solid-phase-enhanced sample preparation (SP3) where we now demonstrate its scalabilit ...[more]

PMID: 31137935

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