Proteomics

Dataset Information

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Sample Preparation by Easy Extraction and Digestion (SPEED) - A Universal, Rapid, and Detergent-free Protocol for Proteomics based on Acid Extraction


ABSTRACT: We report a universal detergent-free method, named Sample Preparation by Easy Extraction and Digestion (SPEED), which consists of three mandatory steps, acidification, neutralization and digestion. SPEED is a universal method for peptide generation from various sources and is easily applicable even for lysis-resistant sample types. The protocol is highly reproducible, virtually loss-less and enables very rapid sample processing. SPEED is superior to detergent/chaotropic agent-based methods for quantitative proteomics and enhances proteome coverage for challenging samples.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bacillus Cereus Atcc 10987 Homo Sapiens (human) Escherichia Coli Staphylococcus Aureus Mus Musculus (mouse)

TISSUE(S): Lung

SUBMITTER: Joerg Doellinger  

LAB HEAD: Peter Lasch

PROVIDER: PXD011189 | Pride | 2019-11-25

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
1077_2017_ZBS6_cProt-09_10Tryp.raw Raw
1078_2017_ZBS6_cProt-09_20Tryp.raw Raw
1079_2017_ZBS6_cProt-09_50Tryp.raw Raw
1080_2017_ZBS6_cProt-09_100Tryp.raw Raw
1081_2017_ZBS6_cProt-025_10Tryp.raw Raw
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