Dataset Information

A rapid methods development workflow for high-throughput quantitative proteomic applications

ABSTRACT: we developed a workflow using data acquired from discovery proteomic experiments, retention time prediction, and standard-flow chromatography to rapidly develop targeted proteomic assays

INSTRUMENT(S): 6550 iFunnel Q-TOF LC/MS

ORGANISM(S): Escherichia Coli

TISSUE(S): Cell Culture

SUBMITTER: Christopher Petzold

LAB HEAD: Christopher J petzold

PROVIDER: PXD011212 | Pride | 2019-06-27

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Agrobacterium tumefaciens.mzid_At_DF-r001_(At-1).pride.mgf.gz	Mzid
	Agrobacterium tumefaciens.mzid_At_DF-r002_(At-2).pride.mgf.gz	Mzid
	Agrobacterium tumefaciens.pride.mztab.gz	Mztab
	Agrobacteriumtumefaciens.mzid.gz	Mzid
	Agrobacteriumtumefaciens.mzid_At_DF-r001_At-1.MGF	Mzid

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Publications

A rapid methods development workflow for high-throughput quantitative proteomic applications.

Chen Yan Y Vu Jonathan J Thompson Mitchell G MG Sharpless William A WA Chan Leanne Jade G LJG Gin Jennifer W JW Keasling Jay D JD Adams Paul D PD Petzold Christopher J CJ

PloS one 20190214 2

Recent improvements in the speed and sensitivity of liquid chromatography-mass spectrometry systems have driven significant progress toward system-wide characterization of the proteome of many species. These efforts create large proteomic datasets that provide insight into biological processes and identify diagnostic proteins whose abundance changes significantly under different experimental conditions. Yet, these system-wide experiments are typically the starting point for hypothesis-driven, fo ...[more]

PMID: 30763335

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