Dataset Information

Investigating Lactococcus lactis MG1363 response to phage p2 infection at the proteome level

ABSTRACT: Phages are viruses that specifically infect and kill bacteria. Bacterial fermentation and biotechnology industries see them as enemies, however, they are also investigated for the treatment or prevention of infections caused by multidrug resistant bacteria. Whether foes or allies, their importance is undeniable. Despite decades of research some aspects of phage biology are still poorly understood. In this study, we used label-free quantitative proteomics to reveal the proteotypes of Lactococcus lactis MG1363 during infection by the virulent phage p2, a model for studying the biology of phages infecting Gram-positive bacteria. Our approach resulted in the high-confidence detection and quantification of 59% of the theoretical bacterial proteome, including 226 bacterial proteins detected only during phage infection and 6 proteins unique to uninfected bacteria. We also identified many bacterial proteins of differing abundance during the infection. Using this high-throughput proteomic datasets, we selected specific bacterial genes for inactivation using CRISPR-Cas9 to investigate their involvement in phage replication. One knockout mutant lacking gene llmg_0219 showed resistance to phage p2 due to a deficiency in phage adsorption. Furthermore, we detected and quantified 78% of the theoretical phage proteome and identified many proteins of phage p2 that had not been previously detected. Among others, we uncovered a conserved small phage protein (ORFN1) coded by an unannotated gene. We also applied a targeted approach to achieve greater sensitivity and identify undetected phage proteins that were expected to be present. This allowed us to follow the fate of ORF46, a small phage protein of low abundance. In summary, this work offers a unique view of the virulent phages’ takeover of bacterial cells and provides novel information on phage-host interactions.

INSTRUMENT(S): LTQ Orbitrap Velos, TSQ Vantage

ORGANISM(S): Lactococcus Phage Lactococcus Lactis Subsp. Cremoris Mg1363

SUBMITTER: Andreas Otto

LAB HEAD: Prof. Dr. Becher

PROVIDER: PXD011263 | Pride | 2019-01-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	150626_V2_PM3_CH_MLL_non_inf_t0_A_1.raw	Raw
	150626_V2_PM3_CH_MLL_non_inf_t0_A_10.raw	Raw
	150626_V2_PM3_CH_MLL_non_inf_t0_A_2.raw	Raw
	150626_V2_PM3_CH_MLL_non_inf_t0_A_3.raw	Raw
	150626_V2_PM3_CH_MLL_non_inf_t0_A_4.raw	Raw

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Investigating <i>Lactococcus lactis</i> MG1363 Response to Phage p2 Infection at the Proteome Level.

Lemay Marie-Laurence ML Otto Andreas A Maaß Sandra S Plate Kristina K Becher Dörte D Moineau Sylvain S

Molecular & cellular proteomics : MCP 20190124 4

Phages are viruses that specifically infect and eventually kill their bacterial hosts. Bacterial fermentation and biotechnology industries see them as enemies, however, they are also investigated as antibacterial agents for the treatment or prevention of bacterial infections in various sectors. They also play key ecological roles in all ecosystems. Despite decades of research some aspects of phage biology are still poorly understood. In this study, we used label-free quantitative proteomics to r ...[more]

PMID: 30679258

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