Dataset Information

Community-level interactions support CH4 oxidation in the Saanich Inlet O2-deficient water column

ABSTRACT: Enrichments with labeled CH4 and NO2 were conducted to test microbial community correlations and constrain potential metabolic interactions between methanotrophs and other one-carbon utilizing microorganisms under low O2 conditions

INSTRUMENT(S): Q Exactive

ORGANISM(S): Environmental Samples

SUBMITTER: Matthew Monroe

LAB HEAD: Stephen Hallam

PROVIDER: PXD011287 | Pride | 2023-01-14

REPOSITORIES: pride

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			Action	DRS
	SH_MetOxInc_Bkgrd_100m_19Feb16_Arwen_16-01-01.mgf	Mgf
	SH_MetOxInc_Bkgrd_100m_19Feb16_Arwen_16-01-01.pride.mgf.gz	Mgf
	SH_MetOxInc_Bkgrd_100m_19Feb16_Arwen_16-01-01.raw	Raw
	SH_MetOxInc_Bkgrd_100m_19Feb16_Arwen_16-01-01_msgfplus_Part1.mzid.gz	Mzid
	SH_MetOxInc_Bkgrd_100m_19Feb16_Arwen_16-01-01_msgfplus_Part1.pride.mztab.gz	Mztab

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Project description:The Baltic Sea is one of the largest brackish water bodies in the world. Redoxclines that form between oxic and anoxic layers in the deepest sub-basins are a semi-permanent character of the pelagic Baltic Sea. The microbially mediated nitrogen removal processes in these redoxclines have been recognized as important ecosystem service that removes large proportion of the nitrogen load originating from the drainage basin. However, nitrification, which links mineralization of organic nitrogen and nitrogen removal processes, has remained poorly understood. To gain better understanding of the nitrogen cycling in the Baltic Sea, we analyzed the assemblage of ammonia oxidizing bacteria and archaea in the central Baltic Sea using functional gene microarrays and measured the biogeochemical properties along with potential nitrification rates. Overall, the ammonia oxidizer communities in the Baltic Sea redoxcline were very evenly distributed. However, the communities were clearly different between the eastern and western Gotland Basin and the correlations between different components of the ammonia oxidizer assemblages and environmental variables suggest ecological basis for the community composition. The more even community ammonia oxidizer composition in the eastern Gotland Basin may be related to the constantly oscillating redoxcline that does not allow domination of single archetype. The oscillating redoxcline also creates long depth range of optimal nitrification conditions. The rate measurements suggest that nitrification in the central Baltic Sea is able to produce all nitrate required by denitrification occurring below the nitrification zone. Two color array (Cy3 and Cy5): the universal standard 20-mer oligo is printed to the slide with a 70-mer oligo (an archetype). Environmental DNA sequences (fluoresced with Cy3) within 15% of the 70-mer conjugated to a 20-mer oligo (fluoresced with Cy5) complementary to the universal standard will bind to the oligo probes on the array. Signal is the ratio of Cy3 to Cy5. Three replicate probes were printed for each archetype. Two replicate arrays were run on duplicate targets.

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Community-level interactions support CH4 oxidation in the Saanich Inlet O2-deficient water column

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