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SWATH-MS proteomics of seeds of healthy and diseased barley

ABSTRACT: Identification and relative quantification of proteins present in seeds of healthy and diseased barley measured by SWATH-MS.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Hordeum Vulgare (barley)

TISSUE(S): Endosperm

SUBMITTER: Edward Kerr

LAB HEAD: Benjamin Luke Schulz

PROVIDER: PXD011303 | Pride | 2018-10-18

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20160630_Barley_IDA.group	Other
	20160630_Barley_IDA_SWATH.swath	Other
	20160630_Barley_IDA_SWATH.xlsx	Xlsx
	20160630_Barley_IDA__FDR.xlsx	Xlsx
	20160701_001_IDA.wiff	Wiff

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Publications

The intrinsic and regulated proteomes of barley seeds in response to fungal infection.

Kerr Edward D ED Phung Toan K TK Caboche Christopher H CH Fox Glen P GP Platz Greg J GJ Schulz Benjamin L BL

Analytical biochemistry 20190608

Barley is an important cereal grain used for beer brewing, animal feed, and human food consumption. Fungal disease can impact barley production, as it causes substantial yield loss and lowers seed quality. We used sequential window acquisition of all theoretical ions mass spectrometry (SWATH-MS) to measure and quantify the relative abundance of proteins within seeds of different barley varieties under various fungal pathogen burdens (ProteomeXchange Datasets PXD011303 and PXD014093). Fungal burd ...[more]

PMID: 31181183

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Transcription profiling of malting barley seeds

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transcriptomic assay on embryos after barley seeds gamma_irradiation-Molecular mechanisms of growth stimulation after low-dose irradiation of barley seeds

Project description:4plex_barley_2017_01 - transcriptomic assay on embryos after barley seeds gamma_irradiation - Why radiation exposure on seeds at low doses causes stimulation of plant growth after germination? - Barley dry seeds of variety “Nur” were irradiated by gamma-rays (Co-60) at doses 20 Gy (stimulatory) and 100 Gy (inhibitory). Immediately after irradiation we put seeds for germination to Petri dishes containing water filter paper; embryos were taken 2h after irradiation, embryos with embrionic roots were taken 24 and 48h after irradiation. The following comparisons have been made: non-irradiated (0 Gy), 2h vs. 20 Gy, 2h; 0 Gy, 24h vs. 20 Gy, 24h; 0 Gy, 48h vs. 20 Gy, 48h; 0 Gy, 24h vs. 100 Gy, 24h. Three independent biological replicates were used.

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Expression data from microdissected endosperm transfer cells (ETCs) of barley seeds during different developmental stages

Project description:Endosperm transfer cells (ETCs) of barley grains/seeds are located at the maternal-filial boundary in seeds and fascilitate nutrient transfer from the mother plant into the filial endosperm tissue. Due to the difficult accessibility of ETCs inside the seed, signalling and metabolic pathways involved in differentiation processes of ETCs are poorly understood. To analyse ETC differentiation, we applied laser microdissection pressure catapulting (LMPC) coupled to transcriptome analysis at various developmental stages from cellularization to full functionality and ongoing modifications. ETC-specific transcriptome data identified candidate genes and associated pathways involved in distinct differentiation processes. Barley grains were harvested at 5, 7, 10 and 12 days after flowering/pollination (DAF) and immediately frozen in liquid nitrogen. In a cryostat (-20M-BM-0C), middle parts were cut by a razor blade, glued onto sample plates by Tissue-TekM-BM-. O.C.TM-bM-^DM-" and 20 M-BM-5m sections were mounted on PEN membrane slides (PALM, Bernried, Germany). Cryosections were stored for 7 days at -20M-BM-0C until complete dryness. LMPC-based isolation of ETC tissue from cryosections has been done using the PALMM-BM-. MicroBeam laser system. For each biological replicate, 50-70 sections from three independent grains were collected. Three biological replicates were generated from each time point (total number of samples = 12).

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Shoot and root of barley seeds: Aluminum treatment

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Shoot and root of barley seeds: Cold treatment

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