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IRES-dependent ribosome repositioning directs translation of a +1 overlapping ORF that enhances viral infection

ABSTRACT: Elucidation of a novel +1 frame translational mechanism from the CrPV intergenic region IRES.

INSTRUMENT(S): 6520A Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Cell Culture

SUBMITTER: Craig Kerr

LAB HEAD: Eric Jan

PROVIDER: PXD011388 | Pride | 2018-11-22

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20170106_CrPV-ORFx_trypsin_01.d.zip	Other
	CrPV-ORFx-Isoforms_DB_082016.fasta	Fasta
	CrPV_ORFx_SearchResults.zip	Other
	CrPV_ProteinDB-Only_04232014.fasta	Fasta
	D_melanogaster_DB_04232014.fasta	Fasta

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Publications

IRES-dependent ribosome repositioning directs translation of a +1 overlapping ORF that enhances viral infection.

Kerr Craig H CH Wang Qing S QS Moon Kyung-Mee KM Keatings Kathleen K Allan Douglas W DW Foster Leonard J LJ Jan Eric E

Nucleic acids research 20181201 22

RNA structures can interact with the ribosome to alter translational reading frame maintenance and promote recoding that result in alternative protein products. Here, we show that the internal ribosome entry site (IRES) from the dicistrovirus Cricket paralysis virus drives translation of the 0-frame viral polyprotein and an overlapping +1 open reading frame, called ORFx, via a novel mechanism whereby a subset of ribosomes recruited to the IRES bypasses 37 nucleotides downstream to resume transla ...[more]

PMID: 30418631

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IRES-dependent ribosome repositioning directs translation of a +1 overlapping ORF that enhances viral infection.

Project description:RNA structures can interact with the ribosome to alter translational reading frame maintenance and promote recoding that result in alternative protein products. Here, we show that the internal ribosome entry site (IRES) from the dicistrovirus Cricket paralysis virus drives translation of the 0-frame viral polyprotein and an overlapping +1 open reading frame, called ORFx, via a novel mechanism whereby a subset of ribosomes recruited to the IRES bypasses 37 nucleotides downstream to resume translation at the +1-frame 13th non-AUG codon. A mutant of CrPV containing a stop codon in the +1 frame ORFx sequence, yet synonymous in the 0-frame, is attenuated compared to wild-type virus in a Drosophila infection model, indicating the importance of +1 ORFx expression in promoting viral pathogenesis. This work demonstrates a novel programmed IRES-mediated recoding strategy to increase viral coding capacity and impact virus infection, highlighting the diversity of RNA-driven translation initiation mechanisms in eukaryotes.

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