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Peptidomics profiling of the murine gut after vertical sleeve gastrectomy

ABSTRACT: Profiling of the murine peptidome along the gastrointestinal tract and pancreas in vertical sleeve gastrectomy and sham operated mice. The study compares the peptidome of various portions of the GI tract using intact mass spectrometry and database searching. These peptides included most gut hormones including proglucagon derived peptides, PYY, GIP, somatostatin, neurotensin amongst others.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Stomach, Colon, Pancreas, Small Intestine

SUBMITTER: Richard Kay

LAB HEAD: Professor Fiona Gribble

PROVIDER: PXD011455 | Pride | 2019-02-09

REPOSITORIES: Pride

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			Action	DRS
	17101306.mgf	Mgf
	17101306.pride.mgf.gz	Mgf
	17101306.raw	Raw
	17101307.mgf	Mgf
	17101307.pride.mgf.gz	Mgf

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Publications

Important Role of the GLP-1 Axis for Glucose Homeostasis after Bariatric Surgery.

Larraufie Pierre P Roberts Geoffrey P GP McGavigan Anne K AK Kay Richard G RG Li Joyce J Leiter Andrew A Melvin Audrey A Biggs Emma K EK Ravn Peter P Davy Kathleen K Hornigold David C DC Yeo Giles S H GSH Hardwick Richard H RH Reimann Frank F Gribble Fiona M FM

Cell reports 20190201 6

Bariatric surgery is widely used to treat obesity and improves type 2 diabetes beyond expectations from the degree of weight loss. Elevated post-prandial concentrations of glucagon-like peptide 1 (GLP-1), peptide YY (PYY), and insulin are widely reported, but the importance of GLP-1 in post-bariatric physiology remains debated. Here, we show that GLP-1 is a major driver of insulin secretion after bariatric surgery, as demonstrated by blocking GLP-1 receptors (GLP1Rs) post-gastrectomy in lean hum ...[more]

PMID: 30726726

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