Dataset Information

Label-free and targeted mass tandem (TMT-labelled) global proteomic analyses on mucus from the stomach, small and large intestine of a domestic and laboratory dogs

ABSTRACT: Gastrointestinal (GI) mucus is continuously secreted and lines the entire length of the GI tract. Essential for health, it keeps the noxious luminal content away from the epithelium and propels forward the digesta. The aim of our study was to characterize the composition and structures of mucus throughout the various GI segments in dog. Mucus from the stomach, small intestine (duodenum, jejunum, ileum), and large intestine (cecum, proximal and distal colon) was collected from 5 dogs. pH and water content of GI mucus and digesta were analyzed. Composition of all GI-tract segments from a domestic and a laboratory dog was determined by label-free global proteomics. A colonic-focussed composition analysis with TMT-labelled proteomics was used on jenunal and proximal and distal colonic mucus samples from 3 laboratory and 1 domestic dog. Finally, the composition of jejunal and colonic mucus samples of 3 laboratory and 1 domestic dog was evaluated with lipidomics and metabolomics. Structural properties were investigated using cryoSEM and rheology. The proteome was similar across the different GI segments. The highest abundant secreted gel-forming mucin in the gastric mucus was mucin 5AC, whether mucin 2 had highest abundance in the intestinal mucus. Lipid and metabolite abundance was generally higher in the jejunal mucus than the colonic mucus. In conclusion, the mucus is a highly viscous and hydrated material. The proteins, lipids and metabolites were similar throughout the GI tract, although abundances depended on location. These data provide an important baseline for future studies on human and canine intestinal diseases and the dog model in drug absorption.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Mucus

SUBMITTER: Xiaofang Cao

LAB HEAD: Janne Lehtiö

PROVIDER: PXD029356 | Pride | 2022-03-07

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20200810_uniprot-proteome_Canis_familiaris_reviewed_no_can.fasta	Fasta
	Metafile_for_dog_paper_-_PROTEOMICS.docx	Other
	XCCBID10-23_20200807_HFTo_dog_mucus_PNGase_labelfree_4hLG_4of20uL_S10.msf	Msf
	XCCBID10-23_20200807_HFTo_dog_mucus_PNGase_labelfree_4hLG_4of20uL_S10.raw	Raw
	XCCBID10-23_20200807_HFTo_dog_mucus_PNGase_labelfree_4hLG_4of20uL_S11.msf	Msf

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Publications

Gastrointestinal mucus in dog: Physiological characteristics, composition, and structural properties.

Dubbelboer Ilse R IR Barmpatsalou Vicky V Rodler Agnes A Karlsson Eva E Nunes Sandro Filipe SF Holmberg Johanna J Häggström Jens J Bergström Christel A S CAS

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 20220225

Gastrointestinal (GI) mucus is continuously secreted and lines the entire length of the GI tract. Essential for health, it keeps the noxious luminal content away from the epithelium. Our aim was to characterize the composition and structure of mucus throughout the various GI segments in dog. Mucus was collected from the stomach, small intestine (duodenum, jejunum, ileum), and large intestine (cecum, proximal and distal colon) from dogs. Composition was determined by multi-omics. Structural prope ...[more]

PMID: 35227857

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