Dataset Information

TACO LEAF, a putative transcriptional regulator in Arabidopsis, is a target of the protein kinase CK2

ABSTRACT: During the growth of multicellular organisms, spatial and temporal patterns of gene expression are strictly regulated to ensure that developmental programs are invoked at the appropriate stage. In this work, we describe a putative transcriptional regulator in Arabidopsis, TACO LEAF (TCO), whose overexpression results in the ectopic activation of reproductive genes during vegetative growth. Isolated as an activation-tagged allele, tco-1D displays gene misexpression patterns and phenotypic abnormalities, such as curled leaves and early flowering, that resemble chromatin regulatory mutants. A role for TCO in this mode of transcriptional regulation is further supported by the subnuclear accumulation patterns of TCO protein and the genetic interactions between tco-1D and mutants of chromatin modifiers. The endogenous expression pattern of TCO and the gene misregulation displayed by tco loss-of-function mutants suggest that this factor is involved in seed development. We also demonstrate that specific serine residues of TCO protein are targeted by the ubiquitous kinase CK2. Collectively, these results identify TCO as a novel regulator of gene expression whose activity is likely influenced by phosphorylation, as is the case with many chromatin regulators

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Danielle Swaney

LAB HEAD: Nevan Krogan

PROVIDER: PXD011565 | Pride | 2019-01-07

REPOSITORIES: Pride

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TCO, a Putative Transcriptional Regulator in Arabidopsis, Is a Target of the Protein Kinase CK2.

Weinman Laina M LM Running Katherine L D KLD Carey Nicholas S NS Stevenson Erica J EJ Swaney Danielle L DL Chow Brenda Y BY Krogan Nevan J NJ Krogan Naden T NT

International journal of molecular sciences 20181228 1

As multicellular organisms grow, spatial and temporal patterns of gene expression are strictly regulated to ensure that developmental programs are invoked at appropriate stages. In this work, we describe a putative transcriptional regulator in Arabidopsis, TACO LEAF (TCO), whose overexpression results in the ectopic activation of reproductive genes during vegetative growth. Isolated as an activation-tagged allele, tco-1D displays gene misexpression and phenotypic abnormalities, suc ...[more]

PMID: 30597831

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Project description:DUX4 is a double homeodomain transcription factor whose misexpression in the muscle causes facioscapulohumeral muscular dystrophy (FSHD). The transcriptional activity of DUX4 has been extensively characterized, and is thought to be the primary driver of FSHD pathogenesis. Yet, DUX4 expression lowers the protein level of an RNA quality control factor, UPF1, without affecting its transcript level, hinting at post-transcriptional regulatory activity downstream of DUX4 expression. How extensive the post-transcriptional activity of DUX4 is, and how relevant it is to FSHD pathogenesis, is unknown. In order to gain insight into DUX4-induced post-transcriptional gene regulation, we measured transcript and protein levels in DUX4 expressing cells via RNA-seq and SILAC-based quantitative mass spectrometry, respectively. We show that DUX4 transcriptional targets are robustly translated, including those genes previously identified as potential FSHD biomarkers. However, comparing the overall pattern of gene expression changes for RNA versus protein reveals striking differences in the most highly activated pathways, with the former showing changes in RNA processing and splicing, and the latter affecting the humoral immune response, proteolysis and exocytosis, among other pathways. Consistent with a misregulation of exocytosis, fluorescence imaging shows fragmented golgi apparatus in DUX4-expressing cells. Of the genes that showed discordant RNA and protein expression levels, post-transcriptional buffering was particularly evident for genes involved in stress response and may explain why DUX4-expressing cells succumb to toxicity despite robust transcriptional activation of stress response genes. Moreover, several genes involved in RNA decay including UPF1, UPF3B, SMG6 and XRN1 showed downregulation at the protein level, which may explain the massive inhibition of RNA quality control in DUX4-expressing cells. These results highlight the importance of considering post-transcriptional gene regulation in DUX4-expressing cells in order to fully understand the FSHD disease process.

			Action	DRS
	NadenKroganTAC.fasta	Fasta
	PhosphoSTYSites.txt	Txt
	SGD_20150113_orf_trans_all.fasta	Fasta
	evidence.txt	Txt
	experimentalDesignTemplate.txt	Txt

Dataset Information

TACO LEAF, a putative transcriptional regulator in Arabidopsis, is a target of the protein kinase CK2

Dataset's files

Publications

TCO, a Putative Transcriptional Regulator in <i>Arabidopsis</i>, Is a Target of the Protein Kinase CK2.

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