Proteomics

Dataset Information

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LC-MSMS of RIBOPROTEOMES OF HEALTHY, POTATO VIRUS A- AND AGROBACTERIUM -INFECTED NICOTIANA BENTHAMIANA PLANTS


ABSTRACT: Nicotiana benthamiana is an important model plant for plant-microbe interaction studies. We compared the proteomes of ribosomes purified from healthy N. benthamiana plants and plants that were infected with two plant pathogens potato virus A (PVA genus Potyvirus) and A. tumefaciens bacteria. Ribosomes were affinity purified from transgenic leaves that expressed FLAG-tagged RPL18B (RPL: ribosome protein large subunit) of A. thaliana. Control purifications were made from non-transgenic plants that were infected with PVA. Our riboproteome revealed ~6600 r-protein hits representing 424 distinct r-proteins that were members from 71 of the expected 81 r-protein families.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Potato Virus A Rhizobium Radiobacter (agrobacterium Tumefaciens) (agrobacterium Radiobacter) Nicotiana Benthamiana

TISSUE(S): Plant Cell, Leaf

DISEASE(S): Viral Infectious Disease

SUBMITTER: Katri Eskelin  

LAB HEAD: Katri Eskelin

PROVIDER: PXD011602 | Pride | 2018-12-05

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
OE1201_5929.raw Raw
OE1201_5929_ARGO1_TR1.pep.xml Pepxml
OE1201_5929_ARGO1_TR1.prot.xml Xml
OE1201_5930.raw Raw
OE1201_5930_ARGO1_TR2.pep.xml Pepxml
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Publications

Ribosome profiles and riboproteomes of healthy and Potato virus A- and Agrobacterium-infected Nicotiana benthamiana plants.

Eskelin Katri K   Varjosalo Markku M   Ravantti Janne J   Mäkinen Kristiina K  

Molecular plant pathology 20181206 3


Nicotiana benthamiana is an important model plant for plant-microbe interaction studies. Here, we compared ribosome profiles and riboproteomes of healthy and infected N. benthamiana plants. We affinity purified ribosomes from transgenic leaves expressing a FLAG-tagged ribosomal large subunit protein RPL18B of Arabidopsis thaliana. Purifications were prepared from healthy plants and plants that had been infiltrated with Agrobacterium tumefaciens carrying infectious cDNA of Potato virus A (PVA) or  ...[more]

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