Proteomics analysis of extracellular matrix remodeling during zebrafish heart regeneration
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ABSTRACT: Adult zebrafish, in contrast to mammals, are able to regenerate their hearts in response to injury or experimental amputation. Our understanding of the cellular and molecular bases that underlie this process, although fragmentary, has increased significantly over the last years. However, the role of the extracellular matrix (ECM) during zebrafish heartregeneration has been comparatively rarely explored. Here, we set out to characterize theECM protein composition inadult zebrafish hearts, and whether it changed during the regenerative response. For this purpose, we first established a decellularization protocol of adult zebrafish ventricles that significantly enriched the yield of ECM proteins. We then performed proteomic analyses of decellularized control hearts and at different times of regeneration. Our results show a dynamic change in ECM protein composition, most evident at the earliest (7 dayspost-amputation) time-point analyzed. Regeneration associated withsharp increases inspecific ECM proteins, and with an overall decrease in collagens and cytoskeletal proteins. We finally tested by atomic force microscopythat the changes in ECM composition translatedto decreased ECM stiffness. Our cumulative results identify changes in the protein composition and mechanical properties of the zebrafish heart ECM during regeneration.
INSTRUMENT(S): Q Exactive
ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)
TISSUE(S): Heart
SUBMITTER:
Joan Josep Bech-Serra
LAB HEAD: Angel Raya
PROVIDER: PXD011627 | Pride | 2019-08-22
REPOSITORIES: Pride
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