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Extracellular Matrix Proteome of Aging-Specific Fibrotic Niche Cells in Mouse Liver Tissue

ABSTRACT: The extracellular matrix (ECM) is a complex network comprising hundreds of proteins, serving not only a structural role in shaping multicellular organisms but also participating in signaling processes. Moreover, the ECM exhibits high tissue specificity and undergoes significant alterations with aging progression. We analyzed the proteome of ECM produced by liver aging-specific fibrotic niche cells in old mice to obtain an age-related proteome profile. Our analysis revealed the identification of 714 quantifiable proteins, with 126 classified as matrisome proteins according to the matrisome database. Notably, proteins significantly upregulated in the ECM of liver aging-specific fibrotic niche cells indicated characteristics reminiscent of ECM formation (Eln, Mfap4, Lox, Tgfb2, Thbs2) and immune cell recruitment (Cxcl12), akin to those originating from fibrotic regions. Our findings suggest that this study may contribute to elucidating heightened fibrotic characteristics associated with the aging process.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver, Fibroblast

SUBMITTER: Narae Park

LAB HEAD: Cheolju Lee

PROVIDER: PXD060715 | Pride | 2025-02-13

REPOSITORIES: pride

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			Action	DRS
	240308_QE_50cm_ECM2_mix2.mzML	Mzml
	240308_QE_50cm_ECM2_mix2.raw	Raw
	240308_QE_50cm_ECM2_mix2_high_cut_analysis.mzTab	Mztab
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